抗pan Cytokeratin (AE1 + AE3)抗体 | Anti-pan Cytokeratin (AE1 + AE3) Antibody

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	Immunohistochemistry-Paraffin: pan Cytokeratin Antibody (AE1 + AE3) [NBP2-29429] - Analysis using Azide and BSA Free version of NBP2-29429. Human Colon Carcinoma stained with pan Cytokeratin Monoclonal Antibody cocktail (AE-1/AE3).
	Immunohistochemistry-Paraffin: pan Cytokeratin Antibody (AE1 + AE3) [NBP2-29429] - Pan Cytokeratin was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using 1.7 ug/mL of mouse anti-pan Cytokeratin monoclonal (NBP2-29429, Novus Biologicals) for 1 hour at room temperature followed by anti-mouse IgG VisUCyte HRP polymer(VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue).
	Flow (Intracellular): pan Cytokeratin Antibody (AE1 + AE3) [NBP2-29429] - An intracellular stain was performed on HeLa cells with pan Cytokeratin Antibody (AE1 + AE3) NBP2-33200PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin. Image from the PE version of this antibody.
	Flow Cytometry: pan Cytokeratin Antibody (AE1 + AE3) [NBP2-29429] - Human Pan-Cytokeratins on HeLa Cells. Black: Cells alone; Green: Isotype Control; Red: PE-labeled Pan-Cytokeratin Monoclonal Antibody (AE-1/AE-3)
	Flow Cytometry: pan Cytokeratin Antibody (AE1 + AE3) [NBP2-29429] - Analysis using Azide and BSA Free version of NBP2-29429. Black: Cells alone; Green: Isotype Control; Red: PE-labeled Pan-Cytokeratin Monoclonal Antibody (AE-1/AE-3).
	Flow (Intracellular): pan Cytokeratin Antibody (AE1 + AE3) [NBP2-29429] - An intracellular stain was performed on HeLa cells with pan Cytokeratin Antibody (AE1 + AE3) NBP2-33200AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647. Image from the Alexa Fluor 647 version of this antibody.

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Background

Keratin/cytokeratin AE1 + AE3 is a pan cytokeratin (also known as pan keratin) antibody cocktail that detects cytokeratins 1-8, 10, 14-16 and 19 (reviewed in Ordonez, 2013). The cocktail is an optimized mixture of two different monoclonal antibody clones, AE1 and AE3. Each clone detects a subset of high and low molecular weight cytokeratins: AE1 detects 10, 14-16, and 19; AE3 detects 1-8. A key advantage of the pan cytokeratin antibody cocktail stain is that a broader spectrum of cytokeratins can be detected compared to using each clone alone. The pan cytokeratin antibody cocktail is a well known broad spectrum immunohistochemical epithelial marker for screening for epithelial differentiation in tumors and their metastases. Most carcinomas have been reported to stain positive, and the antibody can help confirm or rule out the epithelial nature of a poorly differentiated tumor. Positive pan cytokeratin staining with the antibody in the lymph node (Nikura, 2007) or bone marrow ((Berg, 2007) can be an indication of metastatic carcinoma. The pan cytokeratin antibody has also been used to identify residual tumor post-treatment (Azumi, 2006), assess the depth of cancerous invasion (Alexander-Sefre, 2004) and predict survival outcome (Wiedswang, 2004). Negative pan cytokeratin staining patterns can suggest non-epithelial components associated with a carcinoma. For example, ductal lavage foam cells from breast carcinoma patients did not stain with the antibody (Krishnamurthy, 2002). Foam cells are apparently of macrophage origin and hence pan cytokeratin cocktail negative. The pan cytokeratin antibody staining patterns have been extensively documented in epithelium and numerous tumor types, dating back to 1982 (Woodcock-Mitchell) when the clones were first developed. As such, researchers are encouraged to survey the published literature for additional information about pan cytokeratin positive and negative staining tumors as well as in normal tissue.

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