世界最大級の抗体製品数を取り扱うNovus Biologicals社のTLR8 (44C143)に対する抗体(anti-TLR8 (44C143) | antibody TLR8 (44C143))です。Novus Biologicals社の抗体は数多くの学術論文で使用実績があります。
※本製品は研究用です。研究用以外には使用できません。
-Western-Blot-NBP2-24917-img0006.jpg) | Western Blot: TLR8 Antibody (44C143) [NBP2-24917] - analysis of TLR8 in human Ramos cell lysate using this antibody. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test. |
-Immunohistochemistry-Paraffin-NBP2-24917-img0005.jpg) | Immunohistochemistry-Paraffin: TLR8 Antibody (44C143) [NBP2-24917] - Formalin-fixed, paraffin-embedded human spleen stained with TLR8 antibody at 2 ug/ml. |
-Flow-(Intracellular)-NBP2-24917-img0018.jpg) | Flow (Intracellular): TLR8 Antibody (44C143) [NBP2-24917] - Analysis using the PE conjugate of NBP2-24917. Staining of TLR8 in Ramos cells using 2 ug of NBP2-24817. Shaded histogram represents Ramos cells without antibody; green represents isotype control; red represents anti-TLR8 antibody. |
-Flow-Cytometry-NBP2-24917-img0004.jpg) | Flow Cytometry: TLR8 Antibody (44C143) [NBP2-24917] - Intracellular flow analysis of 10^6 human lymphocytes using 1 ug of TLR8 antibody. Shaded histogram represents cells without antibody; green represents isotype control; red represents anti-TLR8 antibody. Goat anti-mouse IgG1 PE conjugate (BD) was used as secondary. |
-Flow-Cytometry-NBP2-24917-img0013.jpg) | Flow Cytometry: TLR8 Antibody (44C143) [NBP2-24917] - Analysis using the FITC conjugate of NBP2-24917. Staining of TLR8 in 10^6 human lymphocytes using 1 ug of this antibody. The shaded histogram represents lymphocytes alone, green represents isotype control, and red represents TLR8 antibody. |
-Flow-(Intracellular)-NBP2-24917-img0014.jpg) | Flow (Intracellular): TLR8 Antibody (44C143) [NBP2-24917] - Analysis using the FITC conjugate of NBP2-24917. Staining of TLR8 in 10^6 Ramos cells using 0.5 ugs of this antibody. The shaded histogram represents Ramos cells alone, green represents isotype control, and red represents TLR8 antibody. |
-Flow-Cytometry-NBP2-24917-img0015.jpg) | Flow Cytometry: TLR8 Antibody (44C143) [NBP2-24917] - Analysis using the PE conjugate of NBP2-24917. Staining of TLR8 in 10^6 human PBMCs using 0.5 ug of NBP2-24817, 0.25 ug of anti-human CD14, and 0.5 ug of isotype control. Novus's TLR intracellular staining flow assay kit was used to test this product. |
-Flow-Cytometry-NBP2-24917-img0016.jpg) | Flow Cytometry: TLR8 Antibody (44C143) [NBP2-24917] - Analysis using the PE conjugate of NBP2-24917. Staining of TLR8 in BALB/c mouse splenocytes (lymphocyte gate) using 2 ug of this antibody. Green represents isotype control ; red represents anti-TLR8 antibody. |
-Flow-Cytometry-NBP2-24917-img0017.jpg) | Flow Cytometry: TLR8 Antibody (44C143) [NBP2-24917] - Analysis using the FITC conjugate of NBP2-24917. Staining of CD14-/CD11c+/TLR8+ myeloid dendritic cells (mDCs). Cells were stained for surface markers CD14 and CD11c, and intracellular stained for TLR8. CD14- cells were gated and stained with FITC-conjugated TLR8 (NBP2-24917), and APC-conjugated CD11c (right). |
-Flow-(Intracellular)-NBP2-24917-img0019.jpg) | Flow (Intracellular): TLR8 Antibody (44C143) [NBP2-24917] - An intracellular stain was performed on A549 cells with TLR8 (44C143) antibody NBP2-24917 (blue) along with a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at RT, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody [F0101B, R&D Systems] ( A). A negative control, HEK293 cells, was also stained to ensure antibody specificity (B). |
-Simple-Western-NBP2-24917-img0007.jpg) | Simple Western: TLR8 Antibody (44C143) [NBP2-24917] - Simple Western lane view shows a specific band for TLR8 in 0.5 mg/ml of Ramos (left) and Human Spleen (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody |