Western Blot: TLR9 Antibody (26C593.2) [Azide Free] [NBP2-24863] - Analysis of TLR9 in A) human PBMC, B) human intestine, C) mouse intestine, and D) rat intestine tissue lysates using this antibody at a dilution of 3 ug/ml.
Immunohistochemistry-Paraffin: TLR9 Antibody (26C593.2) [Azide Free] [NBP2-24863] - Formalin-fixed, paraffin-embedded human spleen probed with TLR9 antibody at 5 ug/ml. Human tissue was used for this test. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Flow Cytometry: TLR9 Antibody (26C593.2) [Azide Free] [NBP2-24863] - Intracellular flow analysis of TLR9 in Ramos cells using 0.5 ug of this antibody. Shaded histogram represents Ramos cells without antibody; green represents isotype control; purple represents anti-TLR9 antibody.
Flow (Intracellular): TLR9 Antibody (26C593.2) - Azide Free [NBP2-24863] - Analysis using the PE conjugate of NBP2-24729. Staining of TLR9 in human PBMCs using 0.2 ug of this antibody. Shaded histogram represents cells without antibody; green represents a mouse IgG1-PE isotype control ; red represents anti-TLR9 antibody.
Flow Cytometry: TLR9 Antibody (26C593.2) - Azide Free [NBP2-24863] - Flow analysis of TLR9 in human PBMC using 0.5 ug of TLR9 antibody (red) and isotype control antibody (green).
Flow Cytometry: TLR9 Antibody (26C593.2) - Azide Free [NBP2-24863] - Intracellular flow cytometric analysis of TLR9 FITC in human B cells using 1ug/10^6 cells of NBP2-24908. Cells were primarily stained with PE-conjugated CD19 antibody. Cells were then fixed and permeabilized and stained with 1ug of either isotype control
Flow Cytometry: TLR9 Antibody (26C593.2) - Azide Free [NBP2-24863] - Analysis using the Azide Free version of NBP2-24729. Staining of TLR9 in Ramos cells using 0.5 ug of this antibody. Shaded histogram represents Ramos cells without antibody; green represents isotype control; purple represents anti-TLR9 antibody.
Flow Cytometry: TLR9 Antibody (26C593.2) - Azide Free [NBP2-24863] - Analysis using the FITC conjugate of NBP2-24729. Staining of TLR9 in Ramos cells using 0.5 ug of this antibody. Green represents isotype control ; red represents anti-TLR9 antibody. TLR intracellular flow kit was used for this test.
Simple Western: TLR9 Antibody (26C593.2) - Azide Free [NBP2-24863] - Simple Western lane view shows a specific band for TLR9 in 0.5 mg/ml of Ramos lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system.