抗LAMP-2/CD107b (H4B4)抗体 | Anti-LAMP-2/CD107b (H4B4) Antibody

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	Genetic Strategies Validation. Knockout Validated: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and LAMP2 knockout (KO) HeLa cell line. PVDF membrane was probed with Mouse Anti-Human LAMP2 Monoclonal Antibody (Catalog # NBP2-22217) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for LAMP2 at approximately 100 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
	Western Blot: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Analysis of LAMP2 expression in HeLa, Jurkat, U937, HepG2 whole cell lysates, human kidney, lung, liver and placenta tissue extracts.
	Immunocytochemistry/Immunofluorescence: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti LAMP-2 (H4B4) [NBP2-22217] at a 1:100 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
	Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - IHC staining of LAMP2 in human liver using DAB with hematoxylin counterstain.
	Flow Cytometry: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - An intracellular stain was performed on SK-MEL-28 cells with LAMP-2/CD107b (H4B4) NBP2-22217AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
	Flow Cytometry: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using LAMP2 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.
	Flow Cytometry: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Analysis using the FITC conjugate of NBP2-22217. Staining of 5ul overlay with isotype on U937 Cells.
	Flow Cytometry: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Using the Alexa Fluor 647 direct conjugate An intracellular stain was performed on HeLa cells with LAMP-2/CD107b (H4B4) NBP2-22217AF647 (blue) and a matched isotype control NBP2-27287AF647 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
	Simple Western: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Simple Western lane view shows a specific band for LAMP2 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

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Background

LAMP2 (Lysosome-associate protein 2) is one of a family of membrane glycoproteins whose function is to provide selectins with carbohydrate ligands. LAMP2 has been implicated in tumor metastasis, as well as overall protection, maintenance and adhesion of the lysosome. Specifically, LAMP2 may protect the lysosomal membrane from auto digestion, and also maintain the acidic environment necessary for the lysosome to function correctly. On a subcellular level, LAMP2 is expressed as a single-pass type I membrane protein, shuttling between endosomes, lysosomes, and the plasma membrane. Mutations in LAMP2 lead to a lysosomal glycogen storage disease known as "Danon Disease". Recently, caspase activation has been suggested in the extracellular export of autophagic vesicles, such as LAMP2 (PMID: 22692030).

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