抗Mucin 5AC (45M1)抗体 | Anti-Mucin 5AC (45M1) Antibody

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	Immunohistochemistry-Paraffin: Mucin 5AC Antibody (45M1) [NBP2-15196] - Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with MUC5AC Monoclonal Antibody (45M1).
	Flow Cytometry: Mucin 5AC Antibody (45M1) [NBP2-15196] - An intracellular stain was performed on A549 cells with Mucin 5AC (45M1) antibody NBP2-15196PE (blue) and a matched isotype control NBP2-27287PE (orange). To block the secretion of Mucin 5AC, cells were treated with 3uM Monensin for 3 hours. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both Antibodies were conjugated to Phycoerythrin.
	Immunohistochemistry-Paraffin: Mucin 5AC Antibody (45M1) [NBP2-15196] - Analysis using the Azide and BSA Free version of NBP2-15196. Detection Human stomach.
	Immunohistochemistry-Paraffin: Mucin 5AC Antibody (45M1) [NBP2-15196] - Formalin-fixed, paraffin-embedded Rat stomach stained with MUC5AC Monoclonal Antibody (45M1).
	Flow Cytometry: Mucin 5AC Antibody (45M1) [NBP2-15196] - Analysis using the Azide and BSA Free version of NBP2-15196. Staining of fixed (70% ethanol or 80% methanol) and permeabilized A549 cells
	Flow (Intracellular): Mucin 5AC Antibody (45M1) [NBP2-15196] - An intracellular stain was performed on A549 cells with Mucin 5AC (45M1) antibody NBP2-15196 (blue) and a matched isotype control NBP2-27287 (orange). To block the secretion of Mucin 5AC, cells were treated with 3uM Monensin for 3 hours. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) PE-conjugated secondary antibody (F0102B, R&D Systems).

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Background

MUC5AC (gastric mucin) belongs to the gel-forming mucin family of glycoproteins, the major components of the protective mucus layer on the mucosal surfaces of epithelial tissues. Mucus protects the tissue surface from mechanical damage, stabilizes the luminal microenvironment, and traps pathogens including bacteria and viruses for mucociliarly clearance. MUC5AC, like other family members, is differentially expressed. MUC5AC is expressed in airway and gastric epithelial cells and highly expressed in colorectal carcinomas. Although not normally detected by antibody in adult colon, MUC5AC is detectable in fetal colon. It is thought that positive MUC5AC antibody staining in colorectal cancer may be due to the resurgence of MUC5AC expression or the unmasking of an embryonic MUC5AC antibody reactive epitope during tumorigenesis. There are qualitative and quantitative alterations of MUC5AC expression in association with various pathological conditions. For example, S dysentariae infection induced MUC5AC expression in HT29 cells which was inhibited by polymyxin B pretreatment (Raja et al, 2011). Antibody studies identified elevated MUC5AC levels in benign and malignant gallbladder lesions (Xiong et al, 2011). On the other hand, MUC5AC expression is decreased or has abnormal glycosylation in the goblet cells of conjunctiva in a number of eye inflammatory conditions including Sjogren, Steven-Johnson and dry eye syndromes (Contreras-Ruiz, 2012). The MUC5AC clone 45M1 antibody is widely used. For example, it has been used as part of an antibody panel to help distinguish esophageal adenocarcinoma (MUC5AC/+) from squamous cell carcinoma (MUC5AC/-) (DiMaio et al, 2012)

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