抗NLRP3/NALP3抗体 | Anti-NLRP3/NALP3 Antibody

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	Western Blot: NLRP3/NALP3 Antibody [NBP2-12446] - Analysis of NALP3 using NALP3 antibody. Lane A) human NALP3 transfected cell lysate this antibody, B) mouse NALP3 transfected cell lysate this antibody, and C) HEK293 control lysate probed with NALP3 antibody at 3 ug/ml.
	Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody [NBP2-12446] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human esophagus using NLRP3/NALP3 antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining in the squamous epithelium was observed. Staining was performed by Histowiz.
	Western Blot: NLRP3/NALP3 Antibody [NBP2-12446] - Analysis of NLRP3 in cells (Huh-7 and SMMC-7721) exposed luteoloside using anti-NLRP3 antibody. Image from verified customer review.
	Western Blot: NLRP3/NALP3 Antibody [NBP2-12446] - Analysis of NALP3 using NALP3 antibody. Human testis lysate in the 1) absence, 2) presence of immunizing peptide, 3) mouse and 4) rat testis probed with NALP3 antibody at 5, 2 and 2 ug/ml respectively. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

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Background

NALP3 (NACHT, LRR and PYD domains-containing protein 3, or NLRP3) belongs to the NLRP family and it contains one DAPIN domain, seven LRR (leucine-rich) repeats and one NATCH domain. It interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain and is a member of the NALP3 inflammasome complex. The NALP3 complex functions as an upstream activator of NF-kappaB signaling and it plays a role in the regulation of inflammation, the immune response and apoptosis. It is also reported that the NALP3 inflammasome can be activated by pathogen-associated molecular patterns (PAMPs) or endogenous danger-associated molecular patterns (DAMPs). The activation of the NALP3 inflammasome results in proteolytic activation and secretion of cytokines of the interleukin-1 (IL-1) family. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome and neonatal-onset multi-system inflammatory disease (NOMID).

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