抗STRO-1 (STRO-1)抗体 | Anti-STRO-1 (STRO-1) Antibody

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	Immunocytochemistry/Immunofluorescence: STRO-1 Antibody (STRO-1) [NBP1-48356] - MG-63 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-STRO-1 (STRO-1) at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
	Flow Cytometry: STRO-1 Antibody (STRO-1) [NBP1-48356] - STRO-1 antibody was tested at 1:400 in MG-63 cells using an Alexa Fluor 488 secondary (shown in green) alongside unstained cells (shown in red).
	Immunocytochemistry/Immunofluorescence: STRO-1 Antibody (STRO-1) [NBP1-48356] - Antibody was tested at 1:50 in MG-63 cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).

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Background

As a part of experimental investigations focused on identifying reliable markers for mesenchymal stromal/stem cells (MSC), STRO-1 antibody was generated by using one of the several hybridomas that were generated by immunizing mice intrasplenically with human CD34+ bone marrow cells and despite lack of much details on exact identity of its cognate antigen, STRO-1 antibody has been heavily relied upon for the recognition/isolation of various types of MSCs for over past two decades. After initial screening against T-/B-cell lines, hybridomas followed further selection for reactivity against subpopulations of CD34-expressing cells as well as for the identification of STRO-1 clone, and additional tests identified STRO-1 antibody as of the IgM isotype which reacts with stromal cells in the adherent layer of long-term bone marrow cultures. STRO-1 has been considered the best-known MSC marker and its antibody has been employed primarily for FLOW application as well as for staining of putative MSCs. However, with the use of IHC and Western blot applications, recent studies have identified STRO-1 as a 75kd endothelial antigen in the vasculature of liver, lung and kidney tissues wherein it co-localized with endothelial marker vWF and also detected it in certain cancer cell lines namely PC3, DU145, MCF7, and K562.

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