抗Vimentin抗体 | Anti-Vimentin Antibody

• 価格
• Image
• Background

価格

目次に戻る

Image

	Western Blot: Vimentin Antibody [NBP1-31327] - Whole cell extracts (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
	Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NBP1-31327] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Vimentin protein stained by Vimentin antibody diluted at 1:500. Blue: Hoechst 33342 staining.
	Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Paraffin-embedded rat ovary. Vimentin antibody diluted at 1:500.
	Western Blot: Vimentin Antibody [NBP1-31327] - A. 20 ug 293T whole cell lysate/extract. B. 10 ug 293T whole cell lysate/extract. C. 5 ug 293T whole cell lysate/extract. D. 1 ug 293T whole cell lysate/extract.
	Western Blot: Vimentin Antibody [NBP1-31327] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody diluted at 1:50000.
	Western Blot: Vimentin Antibody [NBP1-31327] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody diluted at 1:50000.
	Western Blot: Vimentin Antibody [NBP1-31327] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
	Western Blot: Vimentin Antibody [NBP1-31327] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody diluted at 1:2000.
	Genetic Strategies Validation. Western Blot: Vimentin Antibody [NBP1-31327] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody.
	Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NBP1-31327] - Human primary cardiac fibroblasts stained with Vimentin (green) and smooth muscle actin (red) antibodies. Image from verified customer review.
	Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NBP1-31327] - U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Red: Vimentin protein stained by Vimentin antibody diluted at 1:2000. Blue: Hoechst 33342 staining.
	Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Paraffin-embedded U37C xenograft, using antibody at 1:500 dilution.
	Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Paraffin-embedded human lung adenocarcinoma. Vimentin antibody diluted at 1:500.
	Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Analysis of paraffin-embedded RT2 xenograft, using Vimentin antibody at 1:500 dilution.
	Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Paraffin-embedded rat testis. Vimentin antibody diluted at 1:500.
	Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Paraffin-embedded mouse testis. Vimentin antibody diluted at 1:500.
	Immunoprecipitation: Vimentin Antibody [NBP1-31327] - Immunoprecipitation of Vimentin protein from HeLa whole cell extracts using 5ug of Vimentin antibody. Western blot analysis was performed using Vimentin antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.

目次に戻る

Background

Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognising cytokeratins, lymphoid markers, S100, desmin and neurofilaments.

目次に戻る