抗EEA1抗体 | Anti-EEA1 Antibody

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	Western Blot: EEA1 Antibody [NBP1-30914] - A. 30 ug HepG2 whole cell lysate/extract B. 30 ug A375 whole cell lysate/extract 5 % SDS-PAGE EEA1 antibody [C3], C-termdilution: 1:5000
	Immunocytochemistry/Immunofluorescence: EEA1 Antibody [NBP1-30914] - DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: EEA1 protein stained by EEA1 antibody [C3], C-term diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [886] (NBP2-43561) diluted at 1:500. Blue: Fluoroshield with DAPI.
	Immunohistochemistry-Paraffin: EEA1 Antibody [NBP1-30914] - Paraffin-embedded colon carcinoma; antibody dilution 1:500.
	Western Blot: EEA1 Antibody [NBP1-30914] - A: Mouse brain 5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (was used to detect the primary antibody.
	Western Blot: EEA1 Antibody [NBP1-30914] - A. 30 ug PC-12 whole cell lysate/extract, B. 30 ug Rat 2 whole cell lysate/extract 5% SDS-PAGE gel, antibody dilution 1:1000.
	Immunocytochemistry/Immunofluorescence: EEA1 Antibody [NBP1-30914] - Methanol-fixed HeLa, using EEA1 (NBP1-30914) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
	Immunohistochemistry-Paraffin: EEA1 Antibody [NBP1-30914] - Mouse hind brain.
	Immunohistochemistry-Paraffin: EEA1 Antibody [NBP1-30914] - Mouse testis.

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Background

Early endosomal antigen 1 (EEA1) is a 162 kDa membrane bound protein component specific to the early endosome and is essential for fusion between early endocytic vesicles. Early endosomes are cytoplasmic compartments which fuse with endocytic vesicles for redistribution of extracellular compounds to alternate destinations. Zinc finger like domains, reminiscent of those found in nucleic acid binding proteins, are located in the amino and carboxyl terminal domains of EEA1. The carboxyl terminal zinc finger like domain is conserved in several other non nuclear proteins, some of which are also involved in intracellular protein trafficking. In addition, this domain is an authentic zinc binding domain and is critical to the intracellular localization of EEA1. Membrane association of EEA1 has been shown dependent on phosphatidyl 3 kinase activity, inhibitors of which cause EEA1 to dissociate from early endosomes. Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.

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