抗Ferroportin/SLC40A1抗体 | Anti-Ferroportin/SLC40A1 Antibody

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	Western Blot: Ferroportin/SLC40A1 Antibody [NBP1-21502] - Bovine adult eye. Image was submitted via customer review.
	Immunocytochemistry/Immunofluorescence: Ferroportin/SLC40A1 Antibody [NBP1-21502] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-Ferroportin/SLC40A1 conjugated to Alexa Fluor 488 [NBp1-21502AF488] at 20ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
	Immunohistochemistry-Paraffin: Ferroportin/SLC40A1 Antibody [NBP1-21502] - IHC-P detection of FPN1 / SLC40A1 protein in murine small intestinal section using Ferroportin 1 antibody at a dilution of 1:200. The antibody primarily developed a membranous staining pattern in the intestinal epithelial cells.
	Flow (Intracellular): Ferroportin/SLC40A1 Antibody [NBP1-21502] - An intracellular stain was performed on HepG2 cells with Ferroportin/SLC40A1 antibody NBP1-21502AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.
	Western Blot: Ferroportin/SLC40A1 Antibody [NBP1-21502] - Human intestine lysate.
	Immunocytochemistry/Immunofluorescence: Ferroportin/SLC40A1 Antibody [NBP1-21502] - HepG2 cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-Ferroportin 1 at 5.0 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
	Immunohistochemistry-Paraffin: Ferroportin/SLC40A1 Antibody [NBP1-21502] - IHC-P detection of FPN1 / SLC40A1 protein in murine liver section using Ferroportin 1 antibody at a dilution of 1:200. The representative image shows intense staining in the cellular membranes, whereas, a relatively milder postivity was observed in the cytoplasm of hepatocytes.
	Flow Cytometry: Ferroportin/SLC40A1 Antibody [NBP1-21502] - Histogram of Ferroportin staining of human spleen macrophages using Alexa Fluor 700 conjugated Ferroportin antibody (red, catalog# NBP1-21502AF700) and isotype control (grey) as a negative control. Image from verified customer review.
	Flow Cytometry: Ferroportin/SLC40A1 Antibody [NBP1-21502] - Analysis using the PE conjugate of NBP1-21502. Staining of Ferroportin in human B lymphocytes in peripheral blood using PE conjugated anti-Ferroportin antibody. Image from verified customer review.
	Flow Cytometry: Ferroportin/SLC40A1 Antibody [NBP1-21502] - An intracellular stain was performed on HepG2 cells with Ferroportin/SLC40A1 antibody NBP1-21502AF647 (blue) and a matched isotype control NBP2-24893AF647 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

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Background

Ferroportin 1 (FPN1 or SLC40A1) is an iron-regulated transporter (highly expressed in placenta, intestine, muscle, spleen etc.) that play key role in intestinal iron absorption as well as cellular iron release, and mediates iron efflux in the presence of ferroxidases hephaestin (HP) and/or ceruloplasmin (CP). FPN1 is implicated in the iron export from duodenal epithelial cell and also in transfer of iron between maternal and fetal circulation. FPN1 can be regulated transcriptionally, post-transcriptionally, through mRNA stability and post-translationally through protein turnover and its regulation is critical for iron homeostasis because alterations in the same may lead to either iron deficiency or iron overload. FPN1 transports iron in ferrous form whereas plasma transferrin only binds iron's ferric form, and ferroxidases are key players in oxidizing iron transported by FPN1, and without the activity of ferroxidases, FPN1 is internalized followed by degradation. While other cell types in the body utilize the circulating or GPI-linked multicopper ferroxidase CP for FPN1, intestinal cells utilize a membrane-bound HP, a paralog of CP that also show interaction with FPN1. Defects in FPN1 have been linked to hemochromatosis type 4 (HFE4), an autosomal dominant iron-loading disorder.

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