抗TGN38抗体 | Anti-TGN38 Antibody

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	Immunocytochemistry/Immunofluorescence: TGN38 Antibody [NBP1-03495] - Human Melanoma cell line C 32 was cultured overnight on round cover slides placed in a 24 well tissue culture plate. Culture media removed and washed twice with PBS before fixing with 2% formalin for 10 minutes. Cells were then washed three times with PBS and incubated with Tris 0.01M containing Triton X 0.005% for 15 minutes. Cells were washed and incubated with 100 ul of Rabbit antibody to TGN diluted 1:100 in the blocking buffer for 30 minutes. Welles were then washed 7 times with PBS and incubated with 100 ul of anti Rb-FITC conjugate diluted 1:100 in the blocking buffer for further 30 minutes. Cells were washed as before and nuclear counter stained with Hoechst and mounted on to slides.
	Immunocytochemistry/Immunofluorescence: TGN38 Antibody [NBP1-03495] - Human Melanoma cell line C 32.
	Immunocytochemistry/Immunofluorescence: TGN38 Antibody [NBP1-03495] - Human Melanoma cell line C 32
	Immunocytochemistry/Immunofluorescence: TGN38 Antibody [NBP1-03495] - Human Melanoma cell line C 32 was cultured overnight on round cover slides placed in a 24 well tissue culture plate. Culture media removed and washed twice with PBS before fixing with 2% formalin for 10 minutes. Cells were then washed three times with PBS and incubated with Tris 0.01M containing Triton X 0.005% for 15 minutes. Cells were washed and incubated with 100 ul of Rabbit antibody to TGN diluted 1:100 in the blocking buffer for 30 minutes. Welles were then washed 7 times with PBS and incubated with 100 ul of anti Rb-FITC conjugate diluted 1:100 in the blocking buffer for further 30 minutes. Cells were washed as before and nuclear counter stained with Hoechst and mounted on to slides.

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Background

The trans-Golgi network (TGN) is part of the secretory pathway of eukaryotic cells which is distinct from the Golgi stack. The TGN is important in the later stages of protein secretion where it seems to play a key role in the sorting and targeting of secreted proteins to the correct destination. Some surface receptors recycle between the cell surface and the TGN, suggesting that the TGN is also important in endocytic pathways. TGN46 cycles between the trans-Golgi network and the cell surface returning via endosomes. It is thought to be involved in regulating membrane traffic to and from trans-Golgi network. Three splice isoforms are found: TGN46, TGN48 and TGN51. Isoform TGN46 is widely expressed, isoform TGN48 is barely expressed in embryonic kidney and promyelocytic cells whilst isoform TGN51 is more abundant in fetal lung and kidney. TGN46 is a human specific protein. The rodent homologue of TGN46 is known as TGN38.

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