抗PPAR alpha/NR1C1抗体 | Anti-PPAR alpha/NR1C1 Antibody

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Image

	Western Blot: PPAR alpha/NR1C1 Antibody [NBP1-03288] - Affinity Purified Anti-PPAR alpha (N -terminal specific) (Rabbit) is shown to detect a 52 kD band corresponding to PPAR alpha present in a 3T3 whole cell lysate.
	Immunocytochemistry/Immunofluorescence: PPAR alpha/NR1C1 Antibody [NBP1-03288] - PPAR alpha Antibody [NBP1-03288] - Staining of human primary macrophages using NBP1-03288 at a dilution of 1/100. Photo courtesy of a product review by verified customer.
	Immunohistochemistry-Paraffin: PPAR alpha/NR1C1 Antibody [NBP1-03288] - PPAR alpha Antibody [NBP1-03288] - Immunohistochemistry using anti-PPAR antibody, showing staining of PPAR alpha in rat brain sections, highlighting cytoplasmic staining in ependymal cells and neurons in frontal cortex. Bottom image shows subventricular zone (svz) of lateral ventrical (exit point of progenitor olfactory neurones); top image shows frontal cortex in the same section. Cytoplasmic staining is also observed in the corpus callosum (bottom image) and in dendritic fields of the cortex. Formalin/PFA-fixed paraffin-embedded sections of rat brain tissue were incubated with the primary antibody at 1:200 for 1 hour. Antigen retrieval was performed by heat induction in citrate buffer pH 6.0.
	Immunohistochemistry-Paraffin: PPAR alpha/NR1C1 Antibody [NBP1-03288] - Staining of human liver.

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Background

Since their discovery in the early 1990's, the peroxisome proliferator activated receptors (PPARs) have attracted significant attention. This is primarily because PPARs serve as receptors for two very important classes of drugs: the hypolipidemic fibrates and the insulin sensitizing thiazolidinediones. Peroxisome proliferators are non-genotoxic carcinogens that are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed PPARs. Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Upon binding fatty acids or hypolipidemic drugs, PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate the expression of target genes. There are 3 known subtypes of PPARs: PPAR-alpha, PPAR-delta and PPAR-gamma. Mostly target genes are involved in the catabolism of fatty acids. Conversely, PPAR-gamma is activated by peroxisome proliferators such as prostaglandins, leukotrienes and anti-diabetic thiazolidinediones and affects the expression of genes involved in the storage of the fatty acids. PPAR-gamma may also be involved in adipocyte differentiation. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 through interaction with specific response elements.

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