抗FFAR4/GPR120抗体 | Anti-FFAR4/GPR120 Antibody

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	Western Blot: FFAR4/GPR120 Antibody [NBP1-00858] - Analysis of GPR120 (V259) antibody in extracts from LOVO cells.
	Immunocytochemistry/Immunofluorescence: FFAR4/GPR120 Antibody [NBP1-00858] - GPR120 antibody was tested at 1:50 in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.
	Immunohistochemistry-Paraffin: FFAR4/GPR120 Antibody [NBP1-00858] - IHC analysis of formalin fixed paraffin embedded tissue section of mouse intestine using FFAR4/GPR120 antibody at 1:200 dilution. The intestinal epithelial cells showed an expected cytoplasmic staining (immunogen of NBP1-00858 corresponds to the cytoplasmic domain of GPR120 protein ), while some cells especially the goblet cells population depicted nuclear positivity also. The observed nuclear signal may be justified on the fact that - some GPCRs are known for nuclear translocation upon binding to extracellular or endogenous/non-secreted ligands, wherein they impact the trancriptional regulation via GPCR-heterotrimeric G-protein-effector complexes.
	Immunohistochemistry-Paraffin: FFAR4/GPR120 Antibody [NBP1-00858] - IHC analysis of formalin fixed paraffin embedded tissue section of mouse intestine using FFAR4/GPR120 antibody at 1:200 dilution. The intestinal epithelial cells showed an expected cytoplasmic staining (immunogen of NBP1-00858 corresponds to the cytoplasmic domain of GPR120 protein ), while some cells especially the goblet cells population depicted nuclear positivity also. The observed nuclear signal may be justified on the fact that - some GPCRs are known for nuclear translocation upon binding to extracellular or endogenous/non-secreted ligands, wherein they impact the trancriptional regulation via GPCR-heterotrimeric G-protein-effector complexes.

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Background

GPR120, a member of the rhodopsin family of G protein-coupled receptors (GPCRs), is a 377 amino acid protein which is expressed in the intestine. GPR120 is a receptor for unsaturated long-chain FFAs (free fatty acids). FFAs act as signaling molecules and are an important energy source. They also employ various physiological responses through their GPCRs. One such response occurs when dietary FFAs stimulate GPR120. This stimulation promotes the secretion of Glucagon-like peptide-1 (GLP-1) in vivo and in vitro.GLP-1 belongs to the class of molecules known as the incretins, which are associated with insulin secreted from the pancreas as a result of food intake.GLP-1 also inhibits Glucagon and gastric acid secretion and gastric emptying. Consequently, the role of GPR120 in the secretion of GLP-1 is critical in the treatment of diabetes.

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