抗GFP (9F9.F9)抗体 | Anti-GFP (9F9.F9) Antibody

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	Western Blot: GFP Antibody (9F9.F9) [NB600-597] - Lane 1: Molecular Weight Markers. Lane 2: GFP. Load: 10ng per lane. Primary antibody: Ms Anti-GFP antibody at 1:1000 for overnight at 4C. Secondary antibody: Mouse HRP secondary antibody at 1:40,000 for 30 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 27 kDa, 27 kDa for epitope tag GFP. Other band(s): None.
	Immunocytochemistry/Immunofluorescence: GFP Antibody (9F9.F9) [NB600-597] - GFP-transducted 293T cells. Image from verified customer review.
	Western Blot: GFP Antibody (9F9.F9) [NB600-597] - Expression of GFP in CHO-K1 cells trasfected with GFP. Image from verified customer review.
	Western Blot: GFP Antibody (9F9.F9) [NB600-597] - Analysis using the Biotin conjugate of NB600-597. Detection of Green Fluorescent Protein. 50ng of GFP (Lane 1) was run on a 4-20% gel and transferred to 0.45 um nitrocellulose. After blocking with 5% Blotto 30 min at 20 degrees C, Anti-GFP (MOUSE) Monoclonal Antibody BIOTIN Conjugated secondary antibody.
	Western Blot: GFP Antibody (9F9.F9) [NB600-597] - Lane 1: 64pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 2: 32pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 3: 16pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 4: 8pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 5: 4pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 6: 2pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 7: 1g of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 8: 0pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Primary antibody: anti-GFP monoclonal antibody at 1:400 for overnight at 4C. Secondary antibody: HRP-conjugated anti-Mouse IgG was performed at a dilution of 1:20,000 for 1h at 4C. Block: TTBS supplemented with 1% BSA for 1 h at 4C. Predicted/Observed size: 27 kDa for GFP. Other band(s): none.
	Western Blot: GFP Antibody (9F9.F9) [NB600-597] - Recombinantly expressed GFP in Hela. Lane 1: HeLa lysate 50 ng. Lane 2: HeLa lysate 100 ng. Lane 3: HeLa lysate 500 ng. Primary antibody: GFP antibody at 1.0 mg/ml for 1 h at room temperature. Secondary antibody: IRDye 800 conjugated Goat-a-Mouse IgG [H&L] MX10 at 1:2,500 dilution for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 27 kDa, 27 kDa for epitope tag GFP. Other band(s): none.
	Immunocytochemistry/Immunofluorescence: GFP Antibody (9F9.F9) [NB600-597] - Analysis of Biotin conjugate of NB600-597. Used 1:5000 As referenced in: Lu S-M, Tremblay M-E`, King IL, Qi J, Reynolds HM, et al. (2011) HIV-1 Tat-Induced Microgliosis and Synaptic Damage via Interactions between Peripheral and Central Myeloid Cells. PL
	SDS-Page: GFP Antibody (9F9.F9) [NB600-597] - SDS PAGE of Mouse anti-GFP antibody. Lane 1: GFP non-reduced. Lane 2: Molecular Weight Markers. Lane 3: GFP reduced. Load: 10 ug per lane. Other band(s): none.

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Background

Green fluorescence protein (GFP) is a 27 KDa protein derived from the jellyfish Aquorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.

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