抗AKT1 [p Ser473]抗体 | Anti-AKT1 [p Ser473] Antibody

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	Western Blot: Akt1 [p Ser473] Antibody [NB600-590] - Lane 1: AKT1 Recombinant Protein. Lane 2: AKT1 Mutant Human Recombinant Protein. Lane 3: AKT1 (phosphatase treated) Human Recombinant Protein. Load: 50 ng per lane. Primary antibody: AKT pS473 antibody at 1:1,000 for overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT. Predicted/Observed size: 56 kDa, 56 kDa for Gli-2. Other band(s): Gli-2 splice variants and isoforms.
	Immunocytochemistry/Immunofluorescence: Akt1 [p Ser473] Antibody [NB600-590] - Tissue: cardiomyocytes infected with adenovirus expressing with wild-type AKT. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: AKT pS473 antibody at 1:40 for 1 h at RT. Secondary antibody: texas-red conjugated rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: AKT pS473 is nuclear. Staining: AKT pS473 as green fluorescent signal with texas-red conjugated phalloidin (red) to label filamentous actin.
	Immunohistochemistry: Akt1 [p Ser473] Antibody [NB600-590] - Tissue: human breast carcinoma. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Akt pS473 antibody at 100 dilution for 1 h at RT. Secondary antibody: Dako's Techmate streptavidin-biotin reagents at 1:10,000 for 45 min at RT. Localization: Akt pS473 is nuclear and occasionally cytoplasmic.
	Western Blot: Akt1 [p Ser473] Antibody [NB600-590] - Lane 1: nuclear extract from cells infected with adenovirus expressing nuclear-targeted AKT kinase. Load: 35 ug per lane. Primary antibody: AKT pS473 antibody at 1:200 dilution for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 56 kDa for AKT pS473. Other band(s): unspecific.
	Immunohistochemistry-Paraffin: Akt1 [p Ser473] Antibody [NB600-590] - Rabbit anti-AKT pS473 was used at a 1:100 dilution to detect AKT in human breast tumor tissue. The staining is much stronger than the weak basal level of phosphorylation in normal breast.Signal was developed using Dako's Techmate streptavidinbiotin reagents. Tissue was formalin-fixed and paraffin embedded.
	Immunohistochemistry-Paraffin: Akt1 [p Ser473] Antibody [NB600-590] - Rabbit used at a 1:100 dilution to detect AKT in normal human breast tissue. AKT is weakly phosphorylated in normal tissue in the breast. The phosphorylated AKT is clearly localized in the cytoplasm.Signal was developed using Dako's Techmate streptavidin-biotin reagents. Tissue was formalin-fixed and paraffin embedded.

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Background

The serine threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet derived growth factor. The activation is rapid and specific. In the developing nervous system AKT is a critical mediator of growth factor induced neuronal survival. Survival factors can suppress apoptosis in a transcription independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene (referenced from entrez gene).

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