抗Tubulin (YL1/2)抗体 | Anti-Tubulin (YL1/2) Antibody

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	Western Blot: Tubulin Antibody (YL1/2) [NB600-506] - Total protein from human HeLa, mouse 3T3, rat PC12 and African green monkey Cos7 cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with a 1:6000 dilution of anti-Tubulin (YL 1/2) in 1% non-fat milk in TBST and detected with an anti-rat HRP secondary antibody using chemiluminescence.
	Immunocytochemistry/Immunofluorescence: Tubulin Antibody (YL1/2) [NB600-506] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-Tubulin (YL1/2) at a 1:200 dilution overnight at 4C and detected with an anti-rat Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
	Immunohistochemistry-Paraffin: Tubulin Antibody (YL1/2) [NB600-506] - Tubulin antibody was tested in human breast cancer xenograft using DAB with hematoxylin counterstain.
	Flow Cytometry: Tubulin Antibody (YL1/2) [NB600-506] - Analysis of Alexa Fluor (R) 647 conjugate of NB600-506. An intracellular stain was performed on HeLa cells with Tubulin antibody (YL1/2) NB600-506AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with
	Western Blot: Tubulin Antibody (YL1/2) [NB600-506] - Western blot analysis of Tubulin expression in 1) HeLa, 2) NTERA-2, 3) A431, 4) HepG2, 5) MCF7, 6) NIH-3T3, 7) PC-12 and 8) Cos 7 whole cell lysates using NB600-506.
	Western Blot: Tubulin Antibody (YL1/2) [NB600-506] - WB analysis of whole cell lysates of HeLa, NIH-3T3 and PC-12 cell lines using Tubulin antibody (clone YL1/2).
	Immunocytochemistry/Immunofluorescence: Tubulin Antibody (YL1/2) [NB600-506] - Tubulin YL1/2 antibody was tested in HeLa cells with Dylight 550 (red). Nuclei were counterstained with DAPI (blue).
	Immunocytochemistry/Immunofluorescence: Tubulin Antibody (YL1/2) [NB600-506] - Tubulin antibody was tested in HeLa cells with Dylight 550 (red). Nuclei were counterstained with DAPI (blue).
	Immunocytochemistry/Immunofluorescence: Tubulin Antibody (YL1/2) [NB600-506] - Tubulin antibody was tested in HeLa cells with Dylight 550 (red). Nuclei were counterstained with DAPI (blue).
	Immunocytochemistry/Immunofluorescence: Tubulin Antibody (YL1/2) [NB600-506] - B-Tubulin staining (488) and Pericentrin (594) in Mitotic Cell. Image from verified customer review.
	Immunocytochemistry/Immunofluorescence: Tubulin Antibody (YL1/2) [NB600-506] - IF Confocal analysis of NIH/3T3 cells using Tubulin antibody (NB600-506, 1:5). An Alexa Fluor 488-conjugated Goat to rat IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).

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Background

Tubulin alpha-1A chain (TUBA1A), also known as alpha-tubulin 3, tubulin B-alpha-1, B-ALPHA-1, tubulin alpha-3 chain, TUBA3, and LIS3, is a member of the tubulin family. Tubulin is the primary constituent of microtubules. Tubulin binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. The TUBA1A gene is one of three alpha tubulin genes located on chromosome 12q. TUBA1A is found on the cytoskeleton and is expressed predominantly in morphologically differentiated neurologic cells. Mutations to the TUBA1A gene cause a neurological condition called lissencephaly type 3 (LIS3). As a result of defective neuronal migration, LIS3 is characterized by microcephaly, mental retardation, and early-onset epilepsy (PMID: 22264709). The tubulin clone (YL1/2) specifically binds tyrosylated Tubulin (Tyr-Tubulin) (PMID: 6415068).

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