抗beta-Actin (AC-15)抗体 | Anti-beta-Actin (AC-15) Antibody

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	Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - MCDK cells induced with C22H24N2O8 to control the expression of the gene of interest. Beta actin blocking confirms the albumin assay showing that an equal amount of lysate was loaded in each lane.
	Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - beta-Actin expression in Fibroblast Hs68 cell lysate using anti-beta-Actin antibody. Image from verified customer review.
	Simple Western: beta-Actin Antibody (AC-15) [NB600-501] - Analysis using the HRP conjugate of NB600-501. Simple Western lane view shows a specific band for Beta-Actin in 0.2 mg/ml of Hela, MCF-7, SH-SY5Y, & Jurkat lysate(s). This experiment was performed under reducing conditions using the 12-230kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
	Immunocytochemistry/Immunofluorescence: beta-Actin Antibody (AC-15) [NB600-501] - Beta actin was detected in NIH-3T3 cells fixed with methanol using mouse anti-mouse beta-Actin monoclonal antibody (NB600-501) at 1:2700 dilution. Cells were stained using Northern Lights 557 conjugated anti-mouse secondary antibody (NL007) and counterstained with DAPI.
	Immunocytochemistry/Immunofluorescence: beta-Actin Antibody (AC-15) [NB600-501] - HS-68 cells.
	Immunocytochemistry/Immunofluorescence: beta-Actin Antibody (AC-15) [NB600-501] - Immunofluroescence Cultured FS-11 human fibroblast cell line stained using Monoclonal Anti-Beta Actin, Clone No. AC-15 (NB600-501).
	Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - HEK293T cells transfected with Niaph virus Matrix Protein, expression 24h later. This image was submitted via customer review.
	Immunohistochemistry: beta-Actin Antibody (AC-15) [NB600-501] - Breast, Epithelium 40x.
	Flow Cytometry: beta-Actin Antibody (AC-15) [NB600-501] - analysis of HeLa cells using mouse Monoclonal beta-Actin antibody (Orange) and Isotype control Antibody (Blue).
	Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - beta-Actin expression in CHO, GC-1, HeLa, Cos-7 and SH-SY5Y cell lysates using anti-beta-Actin antibody. The primary antibody was used at a dilution of 1:10,000 for 1 hour. Image from verified customer review.
	Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - Analysis of beta-Actin in bovine whole skeletal muscle lysate. Image from verified customer review.
	Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - beta-Actin expression in human cell lines (Caco-2, T84, HCT116, HT29, DU145, BPH1, HeLa). Image from verified customer review.
	Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - Whole cell extract of human fibroblasts was separated on SDS-PAGE and blotted with Monoclonal Anti-beta-Actin. The antibody was developed with Goat Anti-Mouse IgG, Peroxidase conjugate and AEC substrate. Lanes A: Antibody dilution 1:5,000 B: Negative control (only secondary antibody).
	Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - beta-Actin expression in human glioma cells using anti-beta-Actin antibody. Image from verified customer review.
	Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - Analysis of beta-actin in NIH/3T3 and HEK293T cells using HRP conjugated beta-actin antibody (Cat# NB600-501H). Image from verified customer review.
	Immunocytochemistry/Immunofluorescence: beta-Actin Antibody (AC-15) [NB600-501] - Double exposure micrographs of longitudinal, expanded (a,b) and transverse (c) semi-thin cryosections of chicken gizzard muscle double labeled with Monoclonal Anti-alpha-Actin and polyclonal antibodies to chicken gizzard myosin (red). d-f. Longitudinal, expanded cryosections of chicken gizzard muscle double labeled with polyclonal Anti-alpha-Actinin (green, d, e, red, f) in combination with antibodies to chicken gizzard myosin, antibodies to chicken desmin and Monoclonal Anti-Beta Actin,(f, green). Bars, 10 mm. From Dr. J. V. Small, Institute of Molecular Biology, Academy of Sciences, Salzburg.
	Flow Cytometry: beta-Actin Antibody (AC-15) [NB600-501] - Analysis using the HRP conjugate of NB600-501. Electropherogram image(s) of corresponding Simple Western lane view. Beta-Actin antibody was used at 1:500 dilution on Hela, MCF-7, SH-SY5Y, & Jurkat lysate(s).

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Background

Beta-actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. Because beta-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.

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