抗Collagen I alpha 1抗体 | Anti-Collagen I alpha 1 Antibody

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	Western Blot: Collagen I alpha 1 Antibody [NB600-408] - Lane 1: Human Collagen Type 1. Lane 2: None. Load: 50 ng per lane. Primary antibody: Collagen Type I antibody at 1:1,000 overnight at 4C. Secondary antibody: DyLight 649 rabbit secondary antibody at 1:20,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 139 & 130 kDa, 139 & 130 kDa for Collagen Type I. Other Band(s): Collagen Type I splice variants and isoforms.
	Western Blot: Collagen I alpha 1 Antibody [NB600-408] - analysis of Collagen I alpha 1 in porcine burn wound lysate using anti-Collagen I alpha 1 antibody. Image from verified customer review.
	Immunocytochemistry/Immunofluorescence: Collagen I alpha 1 Antibody [NB600-408] - Primary human cardiac fibroblast cells were stained with anti-Collagen I antibody. Cells were cultured for 3 days in DMEM with 10% fetal calf serum. Image from verified customer review.
	Western Blot: Collagen I alpha 1 Antibody [NB600-408] - Detection of collagen I in Wistar rat hepatic stellate cells (HSC) in control (GFP-transduced) (left lane) and PPARg-transduced cell lysates (right lane). Protein staining shown below each blot depicts equal protein loading. An equal amount of the whole cell protein (100 ug) was separated by SDS-PAGE and electroblotted to nitro-cellulose membranes. Proteins were detected by incubating the membrane with anti-Collagen I antibody at a concentration of 0.2-2 ug/10 ml in TBS (100 mM Tris-HCl, 0.15 M NaCl, pH 7.4) with 5% non-fat milk. Detection occurred by incubation with a HRP conjugated secondary antibody at 1 ug/10 ml. Proteins were detected by a chemiluminescent method using the PIERCE ECL kit (Amersham Biosciences).
	Immunocytochemistry/Immunofluorescence: Collagen I alpha 1 Antibody [NB600-408] - Staining of human HaCaT-ras A5-RT3 (SCC) frozen tumor sections. Image provided by Dr. Wa'el Al Rawashdeh of RWTH Aachen University.
	Immunohistochemistry-Paraffin: Collagen I alpha 1 Antibody [NB600-408] - Tissue: right lobe of the liver section. A:Central Vein (CV) fibrosis, B: Non-fibrotic CV, C: Perisinusodial fibrosis, D: Non-fibrotic area, E: Protat tract fibrosis, F: Septal fibrosis (arrow). Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-collagen type I at 1:1250 for 4C for 24hr. Secondary antibody: Peroxidase biotin-streptavidin rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Anti-collagen type I is intra and extracellular. Staining: 3.3'-diaminobenzidine tetrahydrochloride was used as the chromogen. Nuclei were counterstained purple with hematoxylin.
	Immunohistochemistry-Paraffin: Collagen I alpha 1 Antibody [NB600-408] - Rat colon tissue stained with Collagen I alpha 1 antibody (red) and Hoechst (blue). Image from verified customer review.
	Western Blot: Collagen I alpha 1 Antibody [NB600-408] - Cell lysate from human trabecular meshwork. Dilution: 1:1.000. This image was submitted via customer Review.
	Immunocytochemistry: Collagen I alpha 1 Antibody [NB600-408] - Imaging of 4% PFA fixed Feline Adult small intestine. DAPI (blue), pAb (red; Alexa 568). Stained 1:1600 dilution. This image was submitted via customer Review.
	Immunohistochemistry-Paraffin: Collagen I alpha 1 Antibody [NB600-408] - Imaging of rat calvarial defect bone. This image was submitted via customer Review.
	Immunocytochemistry/Immunofluorescence: Collagen I alpha 1 Antibody [NB600-408] - Staining of human dermal fibroblast derived cell sheet. Image provided by product review by verified customer.
	Immunohistochemistry-Paraffin: Collagen I alpha 1 Antibody [NB600-408] - Human lung stained with Collagen I Antibody, diluted at 1:400.
	Immunohistochemistry: Collagen I alpha 1 Antibody [NB600-408] - Analysis of Tissue: human lung. Fixation: formalin fixed paraffin embedded. Antigen retrieval: user optimized. Primary antibody: Collagen I 1:400. Secondary antibody: Peroxidase goat anti-rabbit at 1:10,000 for 45 min at RT. Localization: Strong staining was observed in the extracellular matrix of the lung. Epithelial cells were negative. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.
	Immunohistochemistry-Paraffin: Collagen I alpha 1 Antibody [NB600-408] - Analysis of HRP conjugate of NB600-408. Tissue: Human Skin at pH6. Fixation: formalin fixed paraffin embedded. Primary antibody: Collagen Type I antibody at 10 ug/mL for 1 h at RT. Localization: Collagen Type I is secreted in the extracellular matrix. Sta
	Immunohistochemistry-Paraffin: Collagen I alpha 1 Antibody [NB600-408] - Analysis of HRP conjugate of NB600-408. Tissue: Human Skin at pH9. Fixation: formalin fixed paraffin embedded. Primary antibody: Collagen Type I antibody at 10 ug/mL for 1 h at RT. Localization: Collagen Type I is secreted in the extracellular matrix. Staining: Collagen Type I as precipitated brown signal (A) with hematoxylin purple nuclear counterstain. With corresponding negative conrol (B).

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Background

The pro-alpha 1 subunit of type 1 collagen is encoded by the COL1A1 gene. The collagen type 1 protein is a triple helix composed by one alpha 2 chain and two alpha 1 chains. Type 1 collagen is abundant in bone, dermis, tendon and cornea tissues and is the fibril forming collagen found in the majority of connective tissues. A type of skin tumor called dermatofibrosarcoma protuberans has been linked to reciprocal translocations between the 17th and 22nd chromosomes where the COL1A1 gene is located. Alternate polyadenylation signals result in two transcripts for the collagen type 1 gene. COL3A1 is an important paralog of this gene. Studies using the collagen I alpha 1 antibody show that COL-I degradation is required for tumor growth in certain cancer types where a MT1-MMP complex acts to destroy collagen(1). In a study conducted at the University of Minnesota the collagen I alpha 1 antibody was used to look at the efficacy of using HBOECs (human blood outgrowth endothelial cells) to form tissue-engineered vascular grafts by examining collagen deposition on bioartificial tissue that was either seeded with HBOECs or unseeded(2). Analysis of collagen structure and deposition in tissues using the collagen I alpha 1 antibody has shown that this collagen I alpha 1 antibody is a useful tool in both cancer and wound healing research areas. PMIDs: 1. 22406620 - IHC Staining of Xenografts with Collagen I alpha 1 Antibody 2. 21599543 - Collagen I Alpha 1 Antibody Used in IF Staining of ECM Protein Depositions

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