抗GFP抗体 | Anti-GFP Antibody

• 価格
• Image
• Background

価格

目次に戻る

Image

	Genetic Strategies Validation. Immunocytochemistry/Immunofluorescence: GFP Antibody [NB600-308] - Analysis of GFP in transgenic mouse pancreas in OCT. The blue region in the center is an islet which does not express GFP and is negative. Image from verified customer review.
	Genetic Strategies Validation. Immunocytochemistry/Immunofluorescence: GFP Antibody [NB600-308] - Analysis of GFP in mouse retinal cells. Image courtesy of anonymous customer review.
	Western Blot: GFP Antibody [NB600-308] - analysis of GFP tagged PKA subunits over-expressed in HEK293T cells using GFP antibody. Image from verified customer review.
	Immunocytochemistry/Immunofluorescence: GFP Antibody [NB600-308] - Analysis of GFP in rat liver. Image courtesy of an anonymous customer review.
	Western Blot: GFP Antibody [NB600-308] - Lane 1: 293FT cells transfected with CDK4 dominant negative (C-). Lane 2: 293FT cells positive control (C+). Load: 25 ug per lane. Primary antibody: GFP antibody at 1:400 for overnight at 4C. Secondary antibody: IR Dye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 27 kDa for GFP.
	Western Blot: GFP Antibody [NB600-308] - Wild type GFP (0.1 ug) was used to spike HeLa whole cell lysate. Load: 30 ug per lane. Primary antibody: GFP antibody at 1:1000 for overnight at 4C. Secondary antibody: IR Dye800 Goat-anti-Rabbit IgG [H&L] MX10 at 1:10,000 for 45 min at RT. Block: 5% BLOTTO in PBS overnight at 4C. Predicted/Observed size: 27 kDa for epitope tag GFP. Other band(s): none.
	Western Blot: GFP Antibody [NB600-308] - HEK293T cells expressing Aedes aegypti leucine-rich repeat-containing GPCR (lgr1) fused C-terminally tail with EGFP (139kDa). Lane 1 (untransfected), lane 2 (transient expression) and lane 3 (stable expression). 50ug protein per well. This image was submitted via customer Review.
	Immunohistochemistry-Frozen: GFP Antibody [NB600-308] - Adult mouse brain tissue. This image was submitted via customer review.
	Immunohistochemistry-Paraffin: GFP Antibody [NB600-308] - Staining of GPF+ hepatocytes (brown) transplanted into the liver of a recipient animal using anti-GFP antibody. Cyclin D1 double staining in blue. Image from verified customer review.
	Immunomicroscopy: GFP Antibody [NB600-308] - Monocyte derived dendritic cells and dermal macrophages were challenged and directly visualized with eGFP labeled Dengue virus to localize sequestration of virus particles in the different cells (upper). The location of the GFP was confirmed by TEM (lower magnified view) using rabbit anti GFP Primary antibody (1:200) and a gold labeled secondary antibody. As referenced in: Kwan W-H, Navarro-Sanchez E, Dumortier H, Decossas M, Vachon H, et al. (2008) Dermal-Type Macrophages Expressing CD209/DC-SIGN Show Inherent Resistance to Dengue Virus Growth. PLoS Negl Trop Dis 2(10): e311. doi:10.1371/journal.pntd.0000311
	Immunoprecipitation: GFP Antibody [NB600-308] - IP analysis of GFP in S2 Cells (Drosophila). Image from verified customer review.
	Immunofluorescence: GFP Antibody [NB600-308] - GFP is specifically expressed in vascular pericytes in human glioma stem cell-derived xenografts in mouse brains. The frozen sections were stained with anti-GFP (1:100). This image was submitted via customer Review.

目次に戻る

Background

This GFP antibody has been developed specifically for use in applications requiring a high titer and specificity with minimum background. Polyclonal anti-GFP is designed to detect GFP and its variants. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Polyclonal anti-GFP is designed to detect GFP and its variants. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Anti-GFP can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and can detect GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP containing proteins on western blots.

目次に戻る