Immunocytochemistry/Immunofluorescence: LC3B Antibody [NB600-1384] - LC3B/MAP1 [NB600-1384] - IF Confocal analysis of HeLa cells using LC3B antibody (NB600-1384, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
Immunocytochemistry/Immunofluorescence: LC3B Antibody [NB600-1384] - LC3B/MAP1 [NB600-1384] - LC3 antibody was tested in 50uM Chloroquine treated HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). An antibody concentration of 0.1 ug/ml was used. Image objective 40x.
Biological Strategies Validation. Immunocytochemistry/Immunofluorescence: LC3B Antibody [NB600-1384] - LC3B/MAP1 [NB600-1384] - Analysis using the HRP conjugate of NB600-1384. Staining of treated U373-MG cells using NB600-1384. The nuclei were stained with DAPI.
Western Blot: LC3B Antibody [NB600-1384] - LC3B/MAP1 [NB600-1384] - analysis of LC3B in IB3-1 whole cell lysate using anti-LC3B antibody. Image from verified customer review.
Immunocytochemistry/Immunofluorescence: LC3B Antibody [NB600-1384] - LC3B/MAP1 [NB600-1384] - analysis of LC3B in HeLa cells using anti-LC3B antibody (red). Nuclei were counterstained with DAPI (blue).
Biological Strategies Validation. Western Blot: LC3B Antibody [NB600-1384] - LC3B/MAP1 [NB600-1384] - Detection of LC3B in treated U87-MG (human glioblastoma astrocytoma) lysates.
Biological Strategies Validation. Western Blot: LC3B Antibody [NB600-1384] - Aldosterone (ALD) treated SV-K1 cell. Western blot shows lysates of SV-K1 cell line untreated (-) or treated with aldosterone. This image was submitted via customer review.
Immunocytochemistry/Immunofluorescence: LC3B Antibody [NB600-1384] - Untreated HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-LC3B at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunocytochemistry/Immunofluorescence: LC3B Antibody [NB600-1384] - HeLa cells were treated with 50uM CQ overnight, fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-LC3B at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: LC3B/MAP1LC3B Antibody [NB600-1384] - analysis of U87MG glioma xenografts using anti-LC3B antibdoy. Image from verified customer review.
Simple Western: LC3B/MAP1LC3B Antibody [NB600-1384] - Simple Western lane view shows a specific band for LC3B in 0.5 mg/ml of Neuro2A lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Autophagy is a process of intracellular bulk degradation in which cytoplasmic components, including organelles, are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. During macroautophagy, the sequestering vesicles, termed autophagosomes, fuse with the lysosome or vacuole resulting in the delivery of an inner vesicle (autophagic body) into the lumen of the degradative compartment. There are 16 proteins participating in the autophagy pathway in humans. The autophagy protein LC3, a mammalian homologue of Atg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression may modify MAP1 microtubule-binding activity during development. LC3 is the only known mammalian protein identified that stably associates with the autophagosome membranes. LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction. The detection of LC3-I to LC3-II conversion is a useful and sensitive marker for distinguishing autophagy in mammalian cells.