抗GADD153/CHOP (9C8)抗体 | Anti-GADD153/CHOP (9C8) Antibody

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	Western Blot: GADD153/CHOP Antibody (9C8) [NB600-1335] - Ethanol feeding increases CHOP expression. Image from verified customer review.
	Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (9C8) [NB600-1335] - IHC analysis of a formalin fixed and paraffin embedded (FFPE) tissue section of mouse brain using 1:100 dilution of Calreticulin antibody. The signal was developed using HRP-DAB based detection method which followed counterstaining of the nuclei with hematoxylin. The antibody generated a cytoplasmic and nuclear staining of CHOP in various cell types in the tested section.
	Biological Strategies Validation. Western Blot: GADD153/CHOP Antibody (9C8) [NB600-1335] - Western blot analysis of GADD153/CHOP expression in HeLa cells treated with 2.5ug/ml tunicamycin for 4 hours (Lane 1) and untreated (Lane 2).
	Biological Strategies Validation. Western Blot: GADD153/CHOP Antibody (9C8) [NB600-1335] - Figure 1 shows a Western blot of endogenous CHOP/GADD153 from primary human fibroblasts using NB600-1335. Lane 1: Untreated cells, Lane 2: Cells treated with tunicamycin for 10 hours.
	Western Blot: GADD153/CHOP Antibody (9C8) [NB600-1335] - WB analysis of CHOP in rat heart tissue lysate. Image courtesy of product review submitted by Lee Hsiao-Wei.
	Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (9C8) [NB600-1335] - IHC analysis of a formalin fixed and paraffin embedded (FFPE) tissue section of mouse brain using 1:100 dilution of Calreticulin antibody. The signal was developed using HRP-DAB based detection method which followed counterstaining of the nuclei with hematoxylin. The antibody generated a cytoplasmic and nuclear staining of CHOP in various cell types in the tested section.
	Simple Western: GADD153/CHOP Antibody (9C8) [NB600-1335] - Simple Western lane view shows a specific band for CHOP/GADD153 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

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Background

GADD153, also known as CHOP or DDIT3, is as a growth arrest and DNA damage-inducible gene that encodes a C/EBP-related nuclear protein. This protein functions as a transcriptional inhibitor by forming heterodimers with C/EBP (CCAAT/enhancer-binding protein) and LAP (liver activator protein), and preventing their DNA binding activity. Under normal growth conditions GADD153 is dormant; however it is highly unregulated during cellular stress caused by toxins, metabolic inhibitors and nutrient deprivation. GADD153 is present in the cytosol under nonstressed conditions and then accumulates in the nucleus once it is induced by stress.

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