抗Calreticulin抗体 | Anti-Calreticulin Antibody

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	Western Blot: Calreticulin Antibody [NB600-101] - Human kidney lysate.
	Immunocytochemistry/Immunofluorescence: Calreticulin Antibody [NB600-101] - Immunofluorescence staining of Calreticulin in HCT15 colon cancer cells using NB600-101. Secondary antibody was an Alexa Fluor 488. Photo courtesy of Dr. Birkenkamp Demtroeder, Arhus University Hospital.
	Immunohistochemistry-Paraffin: Calreticulin Antibody [NB600-101] - IHC analysis of a formalin fixed and paraffin embedded (FFPE) tissue section of human thyroid gland using 1:50 dilution of Calreticulin antibody. The signal was developed using HRP-DAB based detection method which followed counterstaining of the nuclei with hematoxylin. This representative section shows a strong positivity of Calreticulin in the follicular epithelial cells, wherein the signal was found to be very intense in the perinuclear region of the cells which correlates well with Endoplasmic reticulum localization of this protein. The para-follicular cells, endothelial cells of blood vessels (not the RBCs though) and the loose connective tissue in the section showed a weak cytoplasmic staining. Some staining was observed in the follicles/colloids also which is potentially the secreted form of Calreticulin.
	Immunocytochemistry/Immunofluorescence: Calreticulin Antibody [NB600-101] - The Calreticulin antibody NB600-101 was tested in HeLa cells at a 1:250 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.
	Immunohistochemistry-Paraffin: Calreticulin Antibody [NB600-101] - IHC analysis of a formalin fixed and paraffin embedded (FFPE) tissue section of human thyroid gland using 1:50 dilution of Calreticulin antibody. The signal was developed using HRP-DAB based detection method which followed counterstaining of the nuclei with hematoxylin. This representative section shows a strong positivity of Calreticulin in the follicular epithelial cells, wherein the signal was found to be very intense in the perinuclear region of the cells which correlates well with Endoplasmic reticulum localization of this protein. The para-follicular cells, endothelial cells and the loose connective tissue in the section showed a weak cytoplasmic staining. Some staining was observed in the follicles/colloids also which is potentially the secreted form of Calreticulin.
	Simple Western: Calreticulin Antibody [NB600-101] - Simple Western lane view shows a specific band for Calreticulin in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

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Background

Calreticulin is a multifunctional, highly conserved Ca2+ -binding protein that is localized to the endoplasmic reticulum (ER). It is also a molecular chaperone and is involved in protein folding events in the ER. Calreticulin has also been shown to interact with the cytoskeleton and to be involved in the regulation of gene expression. Calreticulin may play a role in cellular proliferation including its apparent activity in the proliferation of certain viruses within mammalian host cells. (2-3) Recently it has been shown that calreticulin is induced in response to various types of cell stress including amino acid deprivation. (1) Close interconnections among protein synthesis, gene expression and calcium signaling have been observed by many researchers in recent years. Calreticulin might be centrally located and crucially participate in the coordination of these functions by the cell.(2-3)

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