抗Survivin抗体 | Anti-Survivin Antibody

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	Western Blot: Survivin Antibody [NB500-201] - Whole cell extracts were prepared in RIPA buffer and analyzed using immunoblot analysis. PVDF membrane. Primary antibody 1:1000 at 4C overnight. Secondary antibody 1:20000 at 4C for 1 hour. This image was submitted via customer Review.
	Immunocytochemistry/Immunofluorescence: Survivin Antibody [NB500-201] - Analysis using the HRP conjugate of NB500-201. Staining of Telophase with accumulation of survivin in the midbodies of two daughter cells. Survivin detection using NB500-201.
	Immunohistochemistry-Paraffin: Survivin Antibody [NB500-201] - Survivin shows lysates of human neuroblastoma cell line. PVDF membrane was probed with 1:200 dilution of 0.5 ug/mL of rabbit anti-Survivin polyclonal (NB500-201, Novus Biologicals), followed by 1:2000 dilution of goat anti-rabbit IgG.
	Western Blot: Survivin Antibody [NB500-201] - analysis of Survivin in human hepatocytes from cancer patient (left) and HeLa cell lysate (right) using Survivin antibody. Image from verified customer review.
	Western Blot: Survivin Antibody [NB500-201] - Analysis of Survivin in Hela cell lysate. Image courtesy of anonymous customer review.
	Western Blot: Survivin Antibody [NB500-201] - WB analysis of 30ug of HeLa whole cell lysate (NB800-PC1) using rabbit polyclonal Survivin antibody (NB 500-201) at 1ug/ml. Detection was performed using ECL method with 1 minute exposure.
	Western Blot: Survivin Antibody [NB500-201] - Analysis shows lysates of human neuroblastoma cell line. PVDF membrane was probed with 1:200 dilution of 0.5 ug/mL of rabbit anti-Survivin polyclonal (NB500-201, Novus Biologicals), followed by 1:2000 dilution of goat anti-rabbit IgG.
	Immunocytochemistry/Immunofluorescence: Survivin Antibody [NB500-201] - IF Confocal analysis of HeLa cells using Survivin antibody (NB500-201, 1:10). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
	Immunohistochemistry-Paraffin: Survivin Antibody [NB500-201] - IHC staining of Survivin in human rectal cancer using DAB with hematoxylin counterstain.
	Immunohistochemistry-Paraffin: Survivin Antibody [NB500-201] - analysis of Survivin in ovarian cancer tissue using anti-Survivin antibody. Image from verified customer review.
	Immunohistochemistry: Survivin Antibody [NB500-201] - HRP conjugated Survivin expression in BIRC5 transfected 293T cells. Image from verified customer review.
	Immunohistochemistry: Survivin Antibody [NB500-201] - The top photo is a no primary antibody control stain and the bottom photo is anti-survivin staining of melanoma. Photo courtesy of Dr. Dario Altieri, Yale University.
	Simple Western: Survivin Antibody [NB500-201] - Simple Western lane view shows a specific band for Survivin in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

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Background

Survivin (BIRC5 or IAP4) is the smallest member of inhibitors of apoptosis proteins (X-linked IAP/XIAP, c-IAP1, c-IAP2, IAP-like protein-2/ ILP2, melanoma IAP/ ML-IAP, Livin, NAIP, and survivin) and besides its anti-apoptotic functions; survivin also regulates processes such as cell cycle, migration, metastasis etc. Highly critical in fetal development, survivin is generally no longer present in adult tissues; however, its re-expression occurs in cancers wherein it drives proliferation, metastasis, poor prognosis, and decreased patient survival. Survivin contains only one copy of a modified baculovirus IAP repeat (BIR, a domain required for caspases inactivation) which is used for homodimerization and for its interaction with other chromosome passenger proteins. Phosphorylation/mutation of Thr-48 in the BIR domain affects survivin's dual ability to inhibit apoptosis and regulate cell division. Under apoptotic stimuli involving mitochondria, survivin forms a complex with XIAP, thus increasing the stability of XIAP and its inhibitory activity against caspases. The survivin binding to mitochondrial Smac/DIABLO leads to a delayed Smac/DIABLO release in the cytoplasm resulting in prolonged cell survival. Survivin-HBXIP complex can bind to pro-caspase-9 for exerting prevention of mitochondrial apoptosis activation. For facilitating correct cell division, survivin recognize/complex with phospho-histone-H3 and associates with the chromosome passenger proteins (Aurora B, INCENP, and borealin) and this new complex is recruited to the mitotic centromers where it assists/ensure proper chromosomal segregation. Moreover, at the centrosomes of dividing cells, survivin binds to Cdk1 which is critical for cells to enter mitosis.

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