抗PCNA (PC10)抗体 | Anti-PCNA (PC10) Antibody

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	Western Blot: PCNA Antibody (PC10) [NB500-106] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Histone h3 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
	Immunocytochemistry/Immunofluorescence: PCNA Antibody (PC10) [NB500-106] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-PCNA [PC10] at 5 ug/ml overnight at 4C and detected with an anti-mouse IgG Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
	Immunohistochemistry: PCNA Antibody (PC10) [NB500-106] - Analysis of PCNA in mouse cornea. Image courtesy of product review by Bo-Yie Chen.
	Western Blot: PCNA Antibody (PC10) [NB500-106] - Hela cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-PCNA antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
	Western Blot: PCNA Antibody (PC10) [NB500-106] - Analysis of human (HeLa lysate), murine (SV-T2 lysate), bovine (BAEC lysate), and porcine (PAE lysate) cell extracts.
	Immunohistochemistry-Frozen: PCNA Antibody (PC10) [NB500-106] - IHC analysis of a paraformaldehyde fixed frozen section of brain from murine embryo using PCNA antibody clone PC10 (green), an IB4 antibody (red) and DAPI. Image provided by Dr Siegenthaler via product review.
	Immunohistochemistry-Paraffin: PCNA Antibody (PC10) [NB500-106] - Analysis of paraffin-embedded human colon carcinoma, showing nuclear localization.
	Simple Western: PCNA Antibody (PC10) [NB500-106] - Simple Western lane view shows a specific band for PCNA in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

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Background

PCNA (Proliferating Cell Nuclear Antigen), plays a central role in coordinating the association of replication factors during DNA replication as well as during recognition and repair of DNA damage. PCNA forms a toroidal, ring-shaped structure of 90 kD by the symmetric association of three identical monomers. The ring encircles the DNA, acts as a platform upon which polymerases and other proteins dock to perform various DNA metabolic processes, and functions as a DNA polymerase-delta co-factor. The association of proteins with the replication fork through interactions with PCNA is regulated by several mechanisms. PCNA interacts with numerous proteins to facilitate DNA replication and repair, epigenetic processes and sister chromatid cohesion. PCNA has its highest expression during G1and S-phases, and its expression decreases in G2 and M-phases. This marker is also present in the early G0 phase because of its long half-live of 8-20 h. PCNA is a nuclear nonhistone protein that is necessary for DNA synthesis and is an accessory protein for DNA polymerase alpha, which is elevated during the G1/S phase of the cell cycle. Several studies have been performed to evaluate cell proliferation using PCNA and Ki-67 in different tumors of various origins; compared with PCNA, Ki-67 has been shown to be more sensitive and specific in the various tumors.

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