抗PDI (RL90)抗体 | Anti-PDI (RL90) Antibody

• 価格
• Image
• Background

価格

目次に戻る

Image

	Western Blot: PDI Antibody (RL90) [NB300-517] - Analysis of PDI was performed by loading 25 ug of HepG2 (Lane 1), Hela (Lane 2) and NIH-3T3 (Lane 3) cell lysates.
	Immunocytochemistry/Immunofluorescence: PDI Antibody (RL90) [NB300-517] - Staining in p19 Cells.
	Immunohistochemistry-Paraffin: PDI Antibody (RL90) [NB300-517] - Both normal and cancer biopsies of deparaffinized Human colon tissue tissues.
	Flow Cytometry: PDI Antibody (RL90) [NB300-517] - Analysis of Hela cells compared to an isotype control (blue).
	Western Blot: PDI Antibody (RL90) [NB300-517] - Analysis of PDI expression in 1) human brain and 2) rat liver tissues extracts, 3) HeLa and 4) Hek 293 whole cell lysates.
	Immunocytochemistry/Immunofluorescence: PDI Antibody (RL90) [NB300-517] - Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a PDI monoclonal antibody at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with Dylight 488 goat anti-mouse IgG secondary antibody at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with Dylight 350 Phalloidin at a dilution of 1:120 (2.5units/ml final concentration) and nuclei (red) were stained with DRAQ5 at a concentration of 1ug/ml for 30 minutes.
	Immunocytochemistry/Immunofluorescence: PDI Antibody (RL90) [NB300-517] - Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a PDI monoclonal antibody at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 550 goat anti-mouse IgG secondary antibody at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 488 Phalloidin at a dilution of 1:300 (1 unit/ml final concentration) for 30 minutes.
	Immunocytochemistry/Immunofluorescence: PDI Antibody (RL90) [NB300-517] - Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a PDI monoclonal antibody at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 550 Phalloidin at a dilution of 1:120 (2.5 units/ml final concentration) and nuclei (blue) were stained with Hoechst at a concentration of 1ug/ml for 30 minutes.
	Immunocytochemistry/Immunofluorescence: PDI Antibody (RL90) [NB300-517] - Staining in A549 Cells.
	Immunocytochemistry/Immunofluorescence: PDI Antibody (RL90) [NB300-517] - Staining in HMVEC Cells.
	Immunocytochemistry/Immunofluorescence: PDI Antibody (RL90) [NB300-517] - Staining in NS-1 Cells.
	Immunocytochemistry/Immunofluorescence: PDI Antibody (RL90) [NB300-517] - Staining of Phalloidin (purple) and PDI (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a PDI monoclonal antibody at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 650 Phalloidin at a dilution of 1:120 (2.5 units/ml final concentration) and nuclei (blue) were stained with Hoechst at a concentration of 1ug/ml for 30 minutes.
	Immunohistochemistry-Paraffin: PDI Antibody (RL90) [NB300-517] - Cancer biopsies of deparaffinized Human lung adenocarcinoma tissues.
	Immunohistochemistry-Paraffin: PDI Antibody (RL90) [NB300-517] - Both normal and cancer biopsies of deparaffinized Human tonsil tissues.
	Flow Cytometry: PDI Antibody (RL90) [NB300-517] - Analysis of NIH/3T3 cells compared to an isotype control (blue).
	Flow Cytometry: PDI Antibody (RL90) [NB300-517] - Analysis of K562 cells compared to an isotype control (blue).

目次に戻る

Background

PDI antibody detects protein disulphide-isomerase (PDI) from human, rat, porcine and mouse tissues as well as hamster cells. This antibody has been successfully used in Western blot, immunofluorescence, immunohistochemical, flow cytometry, and immunoprecipitation procedures. By Western blot, this antibody detects a protein at 59 kDa representing PDI from rat liver extract or a slightly higher protein at 61 kDa representing PDI from human liver extract. Immunohistochemical staining of PDI in rat intestine with this antibody yields a pattern consistent with cytoplasmic staining. In immunoprecipitation procedures This antibody has been shown to inhibit the activity of PDI in vitro. This has also been found to inhibit disulfide bond reduction of the HIV protein, gp120, at the cell surface of CHO cells and human lymphoid cells.

目次に戻る