抗AHR (RPT9)抗体 | Anti-AHR (RPT9) Antibody

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	Western Blot: AHR Antibody (RPT9) [NB300-515] - Analysis of Aryl Hydrocarbon Receptor was performed by loading 20 ug of HEK293 lysate overexpressing Aryl Hydrocarbon Receptor (right lane B) or empty vector control (left lane A) and 10ul of PageRuler Plus Prestained Protein Ladder onto a 4-20% Tris-Glycine polyacrylamide gel.
	Immunocytochemistry/Immunofluorescence: AHR Antibody (RPT9) [NB300-515] - Analysis of Aryl Hydrocarbon Receptor using Aryl Hydrocarbon Receptor Monoclonal Antibody (RPT9) shows staining in U251 Cells. Aryl Hydrocarbon Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Aryl Hydrocarbon Receptor at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
	Flow Cytometry: AHR Antibody (RPT9) [NB300-515] - Analysis of Aryl Hydrocarbon Receptor in NIH/3T3 cells compared to an isotype control (blue).
	Western Blot: AHR Antibody (RPT9) [NB300-515] - Analysis of 40 ug of HEK293 lysate overexpressing Aryl Hydrocarbon Receptor (right lane) or empty vector control (left lane) and 10ul of PageRuler Prestained Protein Ladder.
	Immunocytochemistry/Immunofluorescence: AHR Antibody (RPT9) [NB300-515] - Analysis of Aryl Hydrocarbon Receptor using Aryl Hydrocarbon Receptor Monoclonal Antibody (RPT9) shows staining in A2058 Cells. Aryl Hydrocarbon Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Aryl Hydrocarbon Receptor at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
	Immunocytochemistry/Immunofluorescence: AHR Antibody (RPT9) [NB300-515] - Analysis of Aryl Hydrocarbon Receptor using Aryl Hydrocarbon Receptor Monoclonal Antibody (RPT9) shows staining in Hela Cells. Aryl Hydrocarbon Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Aryl Hydrocarbon Receptor at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
	Flow Cytometry: AHR Antibody (RPT9) [NB300-515] - Analysis of Aryl Hydrocarbon Receptor in Hela cells compared to an isotype control (blue).
	Flow Cytometry: AHR Antibody (RPT9) [NB300-515] - Analysis of Aryl Hydrocarbon Receptor in PC-3 cells compared to an isotype control (blue).

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Background

The aryl hydrocarbon receptor (AhR), also known as the dioxin receptor, is a ligand-activated helix/loop/helix transcription factor found in a variety of vertebrate species. The known ligands for AhR are foreign planar aromatic compounds, such as polycyclic aromatic compounds and halogenated aromatic compounds such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Unlike the steroid/thyroid hormone receptors, there is no known physiological ligand for the Ah Receptor. Studies indicate that in non-ligand activated cells, AhR is found complexed with HSP90 predominantly in the cytoplasm. Upon binding to an agonist, the ligand-activated AhR is believed to transform to a nuclear, DNA binding form. This transformation process appears to involve dissociation of HSP90 from AhR followed by formation of a heterodimer with AhR nuclear translocator protein (Arnt). The AhR-ligand complex appears to initiate gene transcription of cytochrome P450 1A1.

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