Western Blot: LRRK2 Antibody [NB300-268] - Detection of LRRK2 in 50 ug of crude bovine brain membrane.
Immunocytochemistry/Immunofluorescence: LRRK2 Antibody [NB300-268] - Mouse CAD cells transfected with Human wild-type LRRK-2 (1:2,000).
Immunohistochemistry-Paraffin: LRRK2 Antibody [NB300-268] - Staining of human brain, Putamen, Neurons and Glia using a 60x magnification.
Flow Cytometry: LRRK2 Antibody [NB300-268] - Analysis using the Alexa Fluor (R) 647 conjugate of NB300-268. Staining of LRRK2 in normal human peripheral blood cells (anticoagulated Lithium-Heparin) using anti-LRRK2 antibody conjugated with AF647. The primary antibody was used at a dilution of 1:200 and incubated for 20 min at room temperature. Image from verified customer.
Western Blot: LRRK2 Antibody [NB300-268] - Detection of LRRK2 in HeLa whole cell lysate (RIPA) using NB300-268. 1:5000 dilution, 1 minute ECL detection.
Immunocytochemistry/Immunofluorescence: LRRK2 Antibody [NB300-268] - LRRK2 antibody was tested in SH-SY-5Y cells at a 1:200 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 568 (Red), and DAPI (Blue), respectively.
Immunohistochemistry-Frozen: LRRK2 Antibody [NB300-268] - Staining as described in PMID 24312256. Image from confirmed customer review.
Immunoprecipitation: LRRK2 Antibody [NB300-268] - Immunoprecipitation of LRRK2 from SH-SY5Y cell lysate followed by an autophosphorylation assay using NB300-268. Lane 1 Rabbit IgG, Lanes 2 and 3 NB300-268.
LRRK2 (leucine-rich repeat serine/threonine-protein kinase 2, also called PARK8 or Dardarin) is a homodimeric multifunctional protein that belongs to TKL serine threonine protein kinases family and LRRK2 immunoreactivity has been documented in neurofibrillary tangles in Alzheimer's disease as well as in PDCG (parkinsonism-dementia complex of Guam). Highly expressed in tissues such as liver, heart, brain etc., LRRK2 localizes to cellular cytoplasm and also associates with membrane structures including mitochondrial outer membrane. LRRK interacts with PARK2, PRDX3 and TPCN2, and phosphorylate proteins which are implicated in Parkinson disease. LRRK2 is a cytoplasmic kinase with autophosphorylation capability as well as GTPase activity. Its interaction with microtubules suggests that LRRK2-induced neurodegeneration might be partly mediated via inhibition of microtubule dynamics, and LRRK2 -mitochondria interactiosn suggests its implication in pathways eliciting ROS mediated damage. LRRK2 has been shown exert positive regulation in autophagy via calcium-sensitive activation of CaMKK/AMPK signaling pathway through activation of NAADP receptors, increase of lysosomal pH as well as calcium release.