抗NF-M抗体 | Anti-NF-M Antibody

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	Immunocytochemistry/Immunofluorescence: 160kDa Neurofilament Medium Antibody [NB300-222] - View of mixed neuron/glial cultures stained with NB300-222 (red). The NF-M protein is assembled into neurofilaments which are found throughout the axons, dendrites and perikarya of these cells.
	NF-M Antibody [NB300-222] - Immunofluorescent analysis of rat cerebellum section stained with chicken pAb to NF-M, NB300-222, dilution 1:1,000 in red, and costained with mouse mAb to CNPase, dilution 1:500, in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free floating sections were stained with above antibodies. The NF-M antibody labels the axons of basket cells and other neurons, while the CNP antibody stains oligodendrocytes, cells that form the myelin sheathes around axons.
	NF-M Antibody [NB300-222] - Western blot analysis of different neuronal tissue and cell lysates using chicken pAb to NF-M, NB300-222, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] NIH/3T3 cells, [7] HEK293, [8] HeLa, [9] SH-SY5Y, and [10] C6 cells. Strong band at 145kDa corresponds to rodent NF-M, and about 160kDa band corresponds to human NF-M protein, visible in SHSY-5Y and HEK293 cells which have neuronal properties. NF-M is not expressed in HeLa and other cell lines tested.

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Background

Neuronal intermediate filaments (NIF) are type-IV intermediate filaments found specifically on neurons where they play key role in maintaining neuronal morphology as well as in regenerating myelinated axons. NIF contain three subunits: a light polypeptide (NEFL/NFL), a medium polypeptide (NEFM/NFM), and a heavy polypeptide (NEFH/NFH), with molecular weights of 68, 160, and 212 kD respectively. Mature NIFs are heteropolymers with about 4:2:1 stoichiometric ratio of NEFL: NEFM: NEFH and also contain a fourth type-IV intermediate filament (IF) subunit, alpha-internexin. NEFM is essential to cross-bridge formation, stabilization, and longitudinal extension of filamentous network and is more critical than NEFH in regulating NIF's structure and function. NEFM is crucial for the acquisition of normal axonal caliber in response to a myelin-dependent "outside-in" trigger for radial axonal growth and removal of the tail domain as well as lysine-serine-proline (KSP) repeats of NEFM, but not NEFH, produces axons with impaired radial growth and reduced conduction velocities. Moreover, mutations of NEFM have been associated with CNS/PNS disorders such as ALS, Parkinson's disease etc., and an imbalance in NEFM's O-GlcNAcylation - phosphorylation regulation plays a crucial role in in Alzheimer disease.

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