抗GAPDH (1D4)抗体 | Anti-GAPDH (1D4) Antibody

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	Western Blot: GAPDH Antibody (1D4) [NB300-221] - analysis of GAPDH in human nucleus pulposus cell culture (40ug/lane) using anti-GAPDH antibody. LI-COR Odyssey imaging system was used for detection. Image from verified customer review.
	Western Blot: GAPDH Antibody (1D4) [NB300-221] - Analysis of cell line lysates probed with mouse mAb to GAPDH, NB300-221, dilution 1:2,000: [1] protein standard, [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS1, [6] NIH-3T3, and [7] C6 cells. The GAPDH antibody reveals a single band at ~37 kDa in all cell lines. GAPDH is a house keeping protein, the level of which is relatively unaffected by most experimental manipulations, and, as a result, this antibody has been widely used as a western blotting loading control.
	Immunocytochemistry/Immunofluorescence: GAPDH Antibody (1D4) [NB300-221] - SH-SY5Y cells stained with GAPDH antibody NB300-221 (green) and Neurofilament Heavy antibody NB300-217 (red), counterstained with a fluorescent DNA probe (blue).
	Western Blot: GAPDH Antibody (1D4) [NB300-221] - analysis of GAPDH in chicken pectoralis major using anti-GAPDH antibody. The primary antibody was used at a dilution of 1:4000, incubated for 1 hour at room temperature. Image from verified customer review.
	Immunocytochemistry/Immunofluorescence: GAPDH Antibody (1D4) [NB300-221] - SH-SY5Y cells stained with GAPDH antibody NB300-221 (green). Nuclear DNA is stained with Hoechst dye (blue).

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Background

GAPDH is a critical enzyme in the glocolytic pathway, catalyzing the 6th step of glycolysis which is the beginning of the pay-off phase of glycolysis where a gain of ATP can be seen. GAPDH is primarily associated with metabolic functions but has also been shown to have a role in transcription activation, apoptosis and intracellular vesicle shuttling. Due to the importance of GAPDH in metabolic processes it is expressed in nearly all cells and therefore serves as a good loading control target. This GAPDH antibody, clone (1D4), is highly validated as a western blot loading control antibody and has been cited in over 180 publications. GAPDH, also known as Glyceraldehyde 3-Phosphate Dehydrogenase is a 146 kDa tetramer composed of four 30-40 kDa subunits and is reported to bind to a variety of other proteins, including the amyloid precursor protein. Associations with Actin and Tubulin have also been reported. The protein may also have a role in the regulation of apoptosis, and interestingly migrates from the cytoplasm into the nucleus when cells become apoptotic. This GAPDH antibody was used as a loading control in a study looking at the effects of RNAi treatment of HCV infected Huh-7 cells on expression levels of various proteins(1). This GAPDH antibody is cited as being used as a loading control in another study looking at the effects of mitogen-activated protein kinases on KChIP2 gene expression(2). The concentration of DPgly and DP in whole cytoplasm, Triton X-100 soluble cytoplasmic fractions and Triton-X 100 insoluble cytoplasmic fractions was analyzed in SCC9 cells at various stages of development; using GAPDH loading control antibody to account for differences in lysate loading(3). The effect of a p38 MAPK inhibitor (SB293063) vs vehicle treatment on the pKv2 phosphorylation state in cortical neurons was studied using this GAPDH antibody as a loading control to account for differences in lysate loading and overall expression levels(4). PMIDs: 1. 12566571: GAPDH Antibody Used as a Loading Control Antibody to Normalize Differences in Lysate Loading. 2. 16385079 Used GAPDH Antibody to Normalize Differences in Overall Expression Levels In Different Patients. 3. 16365169 GAPDH Antibody used as a Loading Control Antibody to Account for Discrepancies in SCC9 Lysate Loading. 4. 17360683 Considers the effect of a p38 MAPK inhibitor on the phosphorylation state of p38 MAPK and the progression of the apoptotic surge of potassium currents using the GAPDH loading control antibody to account for differences in lysate loading.

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