抗Tyrosine Hydroxylase抗体 | Anti-Tyrosine Hydroxylase Antibody

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	Immunocytochemistry/Immunofluorescence: Tyrosine Hydroxylase Antibody [NB300-109] - Dopamine neurons in the mouse substantia nigra. Image from confirmed customer review.
	Immunocytochemistry/Immunofluorescence: Tyrosine Hydroxylase Antibody [NB300-109] - Analysis of Tyrosine Hydroxylase in rat mesenteric artery-whole. Image from verified customer review.
	Immunohistochemistry: Tyrosine Hydroxylase Antibody [NB300-109] - Immunohistochemical staining of retina tissue using TH antibody, NB300-109.
	Immunocytochemistry/Immunofluorescence: Tyrosine Hydroxylase Antibody [NB300-109] - Immunostaining of whole-mount Drosophila brains using NB300-109 at 1:500 dilution. The tyrosine hydroxylase antibody worked really well and produced a bright staining with almost no background. Data courtesy of Dr. Olga Alekseenko, Neurobiology Dept. Harvard Medical School.
	Western Blot: Tyrosine Hydroxylase Antibody [NB300-109] - Western blot of 10 ug of rat striatal lysate showing specificimmunolabeling of the ~60 kDa tyrosine hydroxylase protein
	Simple Western: Tyrosine Hydroxylase Antibody [NB300-109] - Simple Western lane view shows a specific band for Tyrosine Hydroxylase in 0.2 mg/ml of PC-12 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
	Immunocytochemistry: Tyrosine Hydroxylase Antibody [NB300-109] - 10x Magnification cryo-sections mouse: primary antibody: anti-rb TH: 1:500; o.n. secondary antibody: 1:500 dk-anti-rb DyLight488. This image was submitted via customer Review.
	Immunohistochemistry-Paraffin: Tyrosine Hydroxylase Antibody [NB300-109] - Immunohistochemical analysis of a formalin fixed and paraffin embedded rat brain tissue section using Tyrosine Hydroxylase antibody at 1:5000 dilution. The primary antibody binding to its antigen was detected using HRP anti-Polyvalent ready-to-use kit (Ultratek, 35368) with DAB (brown) and the sections were further counterstained using hematoxylin. Isotype control section was incubated with Rabbit IgG Isotype Control antibody and was processed under the same assay conditions. Tyrosine hydroxylase staining (brown) was observed in the test samples only and the signal was specifically localized to the neuronal cells.
	Immunocytochemistry/Immunofluorescence: Tyrosine Hydroxylase Antibody [NB300-109] - Rat midbrain mixed neuronal cultures showing TH positive neurons in green and MAP2 in red. Image courtesy of Aurelie de Rus Jacquet,laboratory of Dr. Jean-Christophe Rochet, Purdue University.

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Background

Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine, epinephrine and norepinephrine. Therefore the regulation of the TH enzyme represents the central means for controlling the synthesis of these important catecholamines. TH has a large molecular diversity, resulting from differential splicing of its mRNA, which is tissue specific and might result in long term changes in activity of the enzyme and its availability of neurotransmitter at various synapses. The presence of different DNA sequences at the TH locus confers susceptibility to various disorders of the brain including manic-depression and schizophrenia. Parkinson's disease is also considered a TH deficiency as low dopamine levels are a consistent neurochemical abnormality.

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