抗Beclin 1抗体 | Anti-Beclin 1 Antibody

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	Western Blot: Beclin 1 Antibody [NB110-87318] - Beclin 1/ATG6 Antibody [NB110-87318] - Detection of Beclin 1 in HeLa whole cell lysate at 1:10,000.
	Western Blot: Beclin 1 Antibody [NB110-87318] - Total protein from human HeLa and mouse Neuro2A cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Beclin-1 in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
	Simple Western: Beclin 1 Antibody [NB110-87318] - Beclin 1/ATG6 Antibody [NB110-87318] - Simple Western lane view shows a specific band for Beclin in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
	Immunocytochemistry/Immunofluorescence: Beclin 1 Antibody [NB110-87318] - Beclin 1/ATG6 Antibody [NB110-87318] - Beclin1 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).
	Immunohistochemistry-Paraffin: Beclin 1 Antibody [NB110-87318] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of human breast cancer using Beclin 1 antibody Lot F4 at 1:300 dilution. The primary antibody bound to Beclin 1 antigen in the tissue section was detected using a HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. This Beclin 1 antibody generated cytoplasmic staining in the cancer as well as the stromal cells. The signal was strongest in a subset of stromal cells which appears to be the cancer associated fibroblasts.
	Immunohistochemistry-Paraffin: Beclin 1 Antibody [NB110-87318] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of human breast cancer using Beclin 1 antibody Lot 71917 at 1:300 dilution. The primary antibody bound to Beclin 1 antigen in the tissue section was detected using a HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. This Beclin 1 antibody generated cytoplasmic staining in the cancer as well as the stromal cells. The signal was strongest in a subset of stromal cells which appears to be the cancer associated fibroblasts.
	Western Blot: Beclin 1/ATG6 Antibody [NB110-87318] - Beclin 1 antibody image from verified customer review.
	Immunohistochemistry: Beclin 1/ATG6 Antibody [NB110-87318] - Staining of normal breast tissue.
	Immunohistochemistry: Beclin 1/ATG6 Antibody [NB110-87318] - Staining of breast carcinoma.

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Background

Beclin 1 is the first identified mammalian gene to mediate autophagy and also has tumor suppressor and antiviral function. Autophagy, a process of bulk protein degradation through an autophagosomic-lysosomal pathway, is important for differentiation, survival during nutrient deprivation, and normal growth control, and is often defective in tumor cells. Beclin 1 was originally isolated in a yeast two hybrid screen to identify Bcl-2-binding partners and maps to a tumor susceptibility locus on human chromosome 17q21 that is frequently monoallelically deleted in human breast, ovarian and prostate cancer. Beclin 1 encodes an evolutionarily conserved 60 kDa coiled coil protein that is expressed in human muscle, epithelial cells and neurons. In gene transfer studies, Beclin 1 promotes nutrient deprivation-induced autophagy, inhibits mammary tumorigenesis, and inhibits viral replication. Expression of the Beclin 1 protein is frequently decreased in malignant breast epithelial cells. Based upon these observations, it is speculated that beclin 1 may work through induction of autophagy to negatively regulate tumorigenesis and to control viral infections. Beclin 1 may also play a role in other biological processes in which autophagy is important such as cell differentiation and nutritional stress responses.

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