抗Hepatic Sinusoidal Endothelial Cells (SE-1)抗体 | Anti-Hepatic Sinusoidal Endothelial Cells (SE-1) Antibody

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	Western Blot: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [NB110-68095] - Total protein from human, mouse and rat liver was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-H.S.E.C in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
	Immunohistochemistry-Frozen: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [NB110-68095] - Immunohistochemical analysis of frozen normal rat liver tissue sections using anti-Hepatic Sinusoidal Endothelial Cells antibody (green). Nuclei were counterstained with DAPI (blue). Image from verified customer review.
	Flow Cytometry: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [NB110-68095] - Using the FITC direct conjugate Flow Cytometry: Surface staining of Rat Liver sinusoidal endothelial cells with Mouse anti-Rat Hepatic Sinusoidal Endothelial Cells [FITC] [NB110-68095F] and negative control. Total viable cells were used for analysis. Image courtesy of customer.

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Background

Hepatic sinusoidal endothelial cells (HSEC) plays a key role in various hepatic pathophysiological conditions and are known for their morphological uniqueness - numerous open fenestrations clustered in groups (sieve plates), lack of distinct basal lamina, and abundant vacuolar/vesicular structures. HSECs are distinct from vascular endothelial cells functionally/morphologically and besides providing sinusoidal barrier, they implicates in the balance or distribution of lipids, cholesterol, and vitamin A, and in the uptake of various substances via their specialized endocytotic mechanisms. SE-1 monoclonal antibody has been specifically designed to recognize an antigen expressed in HSEC, but not in other cell types including vascular endothelial cells. Experiments on developing liver have revealed that the expression of SE-1 antigen is possibly regulated via intimate association of HSECs with hepatocytes. Hepatic sinusoidal endothelial cells (SE-1) antibody has been extensively used as one of the most reliable markers for rat HSEC by Western blot and immunohistochemistry analysis and is a useful tool for studying the role of HSE in various hepato-pathophysiological conditions.

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