抗YAP1抗体 | Anti-YAP1 Antibody

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	Genetic Strategies Validation. Knockout Validated: YAP1 Antibody [NB110-58358] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and YAP1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human YAP1 Polyclonal Antibody (Catalog # NB110-58358) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for YAP1 at approximately 75 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
	Immunocytochemistry/Immunofluorescence: YAP1 Antibody [NB110-58358] - Analysis in Hela cells at a 1:500 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 550 (red) and DAPI (blue), respectively.
	Western Blot: YAP1 Antibody [NB110-58358] - Analysis in transfected HEK 293 cell lysate.
	Simple Western: YAP1 Antibody [NB110-58358] - Simple Western lane view shows a specific band for YAP1 in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.
	Genetic Strategies Validation. Knockout Validated: YAP1 Antibody [NB110-58358] - Western lane view shows lysates of HeLa human cervical epithelial carcinoma parental cell line and YAP1 knockout (KO) HeLa cell line. A specific band was detected for YAP1 at approximately 81 kDa (as indicated) using 50 ug/mL of Rabbit Anti-YAP1 Polyclonal Antibody (Catalog # NB110-58358). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

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Background

YAP1 (Yes-associated protein 1 or Yorkie homolog) is a transcriptional modulator that has been implicated in stem cell differentiation and the regulation of size of organs. It is a transcriptional regulator that can act both as a coactivator as well as a corepressor and is a crucial downstream target of Hippo signaling pathway that plays a pivotal role in restricting cellular proliferation and promoting apoptosis. STK3/MST2, STK4/MST1 and SAV1 activate LATS1/2-MOB1 complex by executing its phosphorylation which in turn phosphorylates and inactivates YAP1 oncoprotein as well as WWTR1/TAZ. LATS1/2 mediated phosphorylation of YAP1 inhibits its translocation into the nucleus to regulate genes responsible for cellular proliferation, death, and migration. TEAD transcription factors are essential for YAP1 to stimulate gene expression, cell growth, anchorage-independent growth, and induction of EMT. YAP1 is frequently overexpressed in certain malignancies including prostate and hepatocellular cancers where it drives the survival of tumors.

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