抗VEGF (EP1176Y)抗体 | Anti-VEGF (EP1176Y) Antibody

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価格

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Image

	Western Blot: VEGF Antibody (EP1176Y) [NB110-57642] - 5 ng of recombinant protein using a 1:1000 dilution.
	Immunocytochemistry/Immunofluorescence: VEGF Antibody (EP1176Y) [NB110-57642] - Staining of HUVEC cells using anti-VEGF.
	Immunohistochemistry-Paraffin: VEGF Antibody (EP1176Y) [NB110-57642] - Mouse kidney tissue sections (paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 5% serum for 45 minutes at 25C. Samples were incubated with primary antibody (1/400 in 4% BSA + 5% serum in PBST) for 14 hours at 4C. An Alexa Fluor 546 conjugated Donkey anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody.
	Flow Cytometry: VEGF Antibody (EP1176Y) [NB110-57642] - Overlay histogram showing HeLa cells stained with VEGF antibody [EP1176Y] - Carboxyterminal end (red line).
	Immunohistochemistry: VEGF Antibody (EP1176Y) [NB110-57642] - Sttaining of human hemangioma using anti-VEGF at a dilution of 1:50.

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Background

Vascular endothelial growth factor (VEGF) was recently identified as a secreted, direct-acting mitogen specific for vascular endothelial cells and capable of stimulating angiogenesis in vivo. Molecular cloning revealed multiple forms of VEGF, apparently arising from alternative splicing of its RNA transcript (1). VEGF and TGF-beta1 are potent angiogenesis inducers with opposing effects on endothelial cells. TGF-beta1 induces apoptosis; VEGF protects endothelial cells from apoptosis. We found that TGF-beta1 promotes endothelial cell expression of FGF-2, which up-regulates VEGF synthesis. Inhibition of VEGF signaling through VEGF receptor 2 (flk-1) abrogates TGF-beta1-induced apoptosis and p38(MAPK) activation. This finding illustrates an unexpected role of VEGF and indicates that VEGF can be pharmacologically converted into an apoptotic factor, a novel approach to antiangiogenesis therapy (2).

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