抗PTP1B/PTPN1 (EP1837Y)抗体 | Anti-PTP1B/PTPN1 (EP1837Y) Antibody

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Image

	Western Blot: PTPN1 Antibody (EP1837Y) [NB110-57442] - Jurkat cell lysate using a 1:200,000 dilution.
	Immunocytochemistry/Immunofluorescence: PTPN1 Antibody (EP1837Y) [NB110-57442] - stained HepG2 cells.
	Immunohistochemistry: PTPN1 Antibody (EP1837Y) [NB110-57442] - Immunohistochemical staining of paraffin-embedded human tonsils using anti-PTP1B (NB110-57442).
	Flow Cytometry: PTPN1 Antibody (EP1837Y) [NB110-57442] - Overlay histogram showing HepG2 cells (red line).

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Background

The non-transmembrane protein tyrosine phosphatase, PTP1B, comprises 435 amino acids, of which the C-terminal 114 residues have been implicated in controlling both localization and function of this enzyme. Increased phosphorylation of PTP1B is seen to accompany the transition from G2 to M phase of the cell cycle. Immunofluorescence experiments indicate that PTP-1B is localized predominantly in the endoplasmic reticulum (ER). Subcellular fractionation is consistent with this localization and establishes that PTP-1B is tightly associated with microsomal membranes, with its phosphatase domain oriented towards the cytoplasm. The finding of a tyrosine phosphatase on the ER suggests new possibilities for cellular events controlled by tyrosine phosphorylation (2). It has also been shown that PTP1B is a novel substrate for Akt and that phosphorylation of PTP1B by Akt at Ser(50) may negatively modulate its phosphatase activity creating a positive feedback mechanism for insulin signaling (3).

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