抗HIF-1 alpha Pack抗体 | Anti-HIF-1 alpha Pack Antibody

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	Western Blot: HIF-1 alpha Antibody Pack [NB100-905] - Analysis of 50ug cobalt chloride induced COS-7 nuclear extracts (NB800-PC26) using NB100-105.
	Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody Pack [NB100-905] - Detection of HIF-1 alpha (red dye 568) in a cultured raw mouse macrophage cell line, using NB100-131. 100X magnification. Photos courtesy of Susan Alexander and Hattie Gresham, PhD.
	Immunohistochemistry-Paraffin: HIF-1 alpha Antibody Pack [NB100-905] - Negative control stain of human placenta (from sea level) using mouse IgG at 1:100. 4uM paraffin-embedded section.
	Flow Cytometry: HIF-1 alpha Antibody Pack [NB100-905] - Analysis of HIF1-alpha in CoCl2 Treated vs. Untreated HeLa Cells. Hela cells were treated for 15 hrs with 200uM CoCl2, fixed in PFA, and permeabilized in 90% MeOH. One million cells were stained with 0.125ug of NB100-449 and secondary FITC-conjugated goat anti-rabbit (in a 150ul reaction). Black- treated, anti-KLH control IgG; Reduntreated, anti-HIF1-alpha; Blue- treated, anti-HIF1-alpha.
	Western Blot: HIF-1 alpha Antibody Pack [NB100-905] - Detection of HIF-1 alpha in Cobalt Chloride treated/untreated Cos-7 nuclear extracts using NB100-654.
	Western Blot: HIF-1 alpha Antibody Pack [NB100-905] - Detection of mouse HIF1-alpha on hypoxia treated MEFs using NB 100-449.
	Western Blot: HIF-1 alpha Antibody Pack [NB100-905] - Detection of HIF-1 alpha in a hypoxic sample. Lane 1: CoCl2 treated Cos-7 nuclear extract (hypoxic). Lane 2: Untreated Cos-7 nuclear extract (normoxic).
	Western Blot: HIF-1 alpha Antibody Pack [NB100-905] - Detection of HIF-1 alpha in a hypoxic sample. Lane 1: CoCl2 treated Cos-7 nuclear extract (50 ug, hypoxic). Lane 2: Untreated Cos-7 nuclear extract (50 ug, normoxic). 10 second ECL exposure. NB100-479.
	Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody Pack [NB100-905] - Detection of HIF-1 alpha (red dye) in a cell cytospin from a lavage of a murine skin pouch infected with Staph Aureus, using NB100-131. Blue dye is DAPI nuclear staining. Photos courtesy of Susan Alexander and Hattie Gresham, PhD.
	Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody Pack [NB100-905] - Detection of HIF-1 alpha (red dye) in a cell cytospin from a lavage of a murine skin pouch infected with Staph Aureus, using NB100-131. 100X magnification. Blue dye is DAPI nuclea
	Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody Pack [NB100-905] - Detection of HIF-1 alpha (red dye 568) in a cultured raw mouse macrophage cell line, using NB100-131. Photos courtesy of Susan Alexander and Hattie Gresham, PhD
	Immunohistochemistry: HIF-1 alpha Antibody Pack [NB100-905] - Staining of mouse brain, neurons using NB100-449.
	Immunohistochemistry: HIF-1 alpha Antibody Pack [NB100-905] - Staining of placenta, villi using NB100-479.
	Immunohistochemistry: HIF-1 alpha Antibody Pack [NB100-905] - Immunohistochemical staining of human glioblastoma multiforme utilizing NB100-105.
	Immunohistochemistry: HIF-1 alpha Antibody Pack [NB100-905] - HIF-1 Alpha staining in hypoxia-induced human placenta using NB 100-131.

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Background

Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein that activates gene transcription in response to reduced cellular 02 concentration. HIF-1 activates the transcription of EPO, VEGF, iNOS, heme oxygenase 1 and several other critical intracellular responses to hypoxia. HIF-1 is a heterodimer composed of HIF-1a and HIF-1b subunits. Both subunits are induced by hypoxia and rapidly decay upon return to normoxia and are basic-helix-loop-helix-PAS proteins. Recent research indicates the ability to regulate hypoxia-inducible factors may be related to tumor-related angiogenesis in certain cancers.

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