Western Blot: HIF Prolyl Hydroxylases Antibody Pack [NB100-903PHD] - Analysis of human PHD2. Samples: Recombinant FLAG-His-PHD1, PHD2 or PHD3 (10ng/lane) and whole cell lysate from HeLa cells.
Immunohistochemistry: HIF Prolyl Hydroxylases Antibody Pack [NB100-903PHD] - Staining of renal tubular epithelium in mouse at 2.5ug/ml. Mouse kidney cortex, 40X.
Flow Cytometry: HIF Prolyl Hydroxylases Antibody Pack [NB100-903PHD] - Detection of PHD2. 1 million Jurkat cells were fixed, permeabilized, and stained with 3.0 ug/ml anti-PHD2 NB100-137 in a 150 ul reaction. Isotype control (black), anti-MLL1 (red).
Western Blot: HIF Prolyl Hydroxylases Antibody Pack [NB100-903PHD] - Analysis of human PHD2. Samples: Recombinant FLAG-His-PHD1, PHD2 and PHD3 (10 ng/lane), HeLa whole cell lysate and MEFs.
Western Blot: HIF Prolyl Hydroxylases Antibody Pack [NB100-903PHD] - Detection of Human and Rat PHD3 by Western Blot. Samples: A. Lysate from cells that were transiently transfected with PHD1, PHD2, PHD3 or PHD4 constructs or in vitro translated PHD1, PHD2, PHD3, PHD4. B. Lysate from rat cells before or 9 hours after inducing hypoxia. Antibody: Affinity purified rabbit anti-PHD3 (NB100-139) use at 1:1,000 (A) or 1:5,000 (B). Detection: Chemiluminence.
Western Blot: HIF Prolyl Hydroxylases Antibody Pack [NB100-903PHD] - Analysis of human PHD4 Samples: whole cell lysate from HeLa cells, normoxic and hypoxic, respectively.
Western Blot: HIF Prolyl Hydroxylases Antibody Pack [NB100-903PHD] - Analysis of human PHD3. Samples: HeLa whole cell lysates and MEFs.
Western Blot: HIF Prolyl Hydroxylases Antibody Pack [NB100-903PHD] - Analysis of human PHD.
Western Blot: HIF Prolyl Hydroxylases Antibody Pack [NB100-903PHD] - Detection of PHD2 in mouse kidney lysate. ECL exposure, 20 seconds.