抗HIF-1 alpha Pack抗体 | Anti-HIF-1 alpha Pack Antibody

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	Western Blot: HIF-1 alpha Antibody Pack [NB100-901IHC] - Detection of mouse HIF1-alpha on hypoxia treated MEFs using NB 100-449.
	Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody Pack [NB100-901IHC] - Detection of HIF-1 alpha (red dye 568) in a cultured raw mouse macrophage cell line, using NB100-131. 100X magnification. Photos courtesy of Susan Alexander and Hattie Gresham, PhD.
	Immunohistochemistry: HIF-1 alpha Antibody Pack [NB100-901IHC] - Staining of placenta, villi using.
	Flow Cytometry: HIF-1 alpha Antibody Pack [NB100-901IHC] - Analysis of HIF1-alpha in CoCl2 Treated vs. Untreated HeLa Cells. Hela cells were treated for 15 hrs with 200uM CoCl2, fixed in PFA, and permeabilized in 90% MeOH. One million cells were stained with 0.125ug of NB100-449 and secondary FITC-conjugated goat anti-rabbit (in a 150ul reaction). Black- treated, anti-KLH control IgG; Reduntreated, anti-HIF1-alpha; Blue- treated, anti-HIF1-alpha.
	Western Blot: HIF-1 alpha Antibody Pack [NB100-901IHC] - Detection of HIF-1 alpha in cobalt chloride treated/untreated COS-7 nuclear extracts.
	Western Blot: HIF-1 alpha Antibody Pack [NB100-901IHC] - On day 1, MEF cells (+/+,-/-), were grown on 15cm dish (2x10 to the 6th cells). On day 2, cells were exposed to hypoxia for 4hrs. Cells were washed with ice cold PBS twice and whole cell protein was extracted with RIPA buffer fortified with protease. Upon quantification, 100ug of protein was fractionated on 7% polyacralymide gel. -Gel was transferred overnight onto nitrocellulose membrane. The membrane was probed with HIF-1 alpha monoclonal antibody at a 1:500 dilution (NB 100-123). The secondary antibody was conjugated with HRP and was used at a 1:2500 dilution. Photo courtesy of Dr. Gregg Semenza, Johns Hopkins University.
	Western Blot: HIF-1 alpha Antibody Pack [NB100-901IHC] - Detection of HIF-1 alpha in a hypoxic sample. Lane 1: CoCl2 treated Cos-7 nuclear extract (hypoxic). Lane 2: Untreated Cos-7 nuclear extract (normoxic) NB100-449.
	Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody Pack [NB100-901IHC] - Detection of HIF-1 alpha (red dye) in a cell cytospin from a lavage of a murine skin pouch infected with Staph Aureus, using NB100-131. Blue dye is DAPI nuclear staining. Photos courtesy of Susan Alexander and Hattie Gresham, PhD.
	Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody Pack [NB100-901IHC] - Detection of HIF-1 alpha (red dye) in a cell cytospin from a lavage of a murine skin pouch infected with Staph Aureus, using NB100-131. 100X magnification. Blue dye is DAPI nuclear staining. Photos courtesy of Susan Alexander and Hattie Gresham, PhD.
	Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody Pack [NB100-901IHC] - Detection of HIF-1 alpha (red dye 568) in a cultured raw mouse macrophage cell line. Photos courtesy of Susan Alexander and Hattie Gresham, PhD.
	Immunohistochemistry: HIF-1 alpha Antibody Pack [NB100-901IHC] - Staining of HIF-1 alpha in human kidney.
	Immunohistochemistry: HIF-1 alpha Antibody Pack [NB100-901IHC] - Staining in hypoxia-induced human placenta.
	Immunohistochemistry-Paraffin: HIF-1 alpha Antibody Pack [NB100-901IHC] - Negative control stain of human placenta (from sea level) using mouse IgG at 1:100. 4uM paraffin-embedded section.
	Immunohistochemistry: HIF-1 alpha Antibody Pack [NB100-901IHC] - Staining of mouse brain, neurons using NB100-449.
	Immunoprecipitation: HIF-1 alpha Antibody Pack [NB100-901IHC] - Immunoprecipitation of HIF-1alpha using NB100-123. Heavy and light chains are also detected.

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Background

Our HIF-1 alpha sample pack provides a convenient way to determine the optimal HIF-1 alpha antibody for your specific species and tissues. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein that activates gene transcription in response to reduced cellular 02 concentration. HIF-1 activates the transcription of EPO, VEGF, iNOS, heme oxygenase 1 and several other critical intracellular responses to hypoxia. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are induced by hypoxia and rapidly decay upon return to normoxia and are basic-helix-loop-helix-PAS proteins. Recent research indicates the ability to regulate hypoxia-inducible factors may be related to tumor-related angiogenesis in certain cancers.

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