抗MHC Class II RT1B (OX-6)抗体 | Anti-MHC Class II RT1B (OX-6) Antibody

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	Western Blot: MHC Class II RT1B Antibody (OX-6) [NB100-65541] - Total protein from mouse placenta and spleen was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-RT1b in 1% milk, and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
	Immunohistochemistry-Paraffin: MHC Class II RT1B Antibody (OX-6) [NB100-65541] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse spleen using MHC Class II RT1B antibody (clone OX-6) at 1:100 dilution. The signal was developed using HRP-labelled secondary antibody and DAB reagent, and the nuclei were counterstained with hematoxylin. The antibody generated very specific signal in a subset of spleenocytes.
	Flow Cytometry: MHC Class II RT1B Antibody (OX-6) [NB100-65541] - Analysis using Alexa Fluor (R) 488 conjugate of NB100-65541. Rat Splenocytes were stained with MHC Class II RT1B (OX-6) antibody NB100-65541 (blue) and a matched isotype control NBP2-27287 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. The antibodies were directly conjugated to Alexa Fluor 488.
	Western Blot: MHC Class II RT1B Antibody (OX-6) [NB100-65541] - Rat Spleen Tissue lysate probed with MHC Class II antibody.
	Flow Cytometry: MHC Class II RT1B Antibody (OX-6) [NB100-65541] - Staining of rat splenocytes with Mouse anti Rat MHC class II RT1B
	Flow Cytometry: MHC Class II RT1B Antibody (OX-6) [NB100-65541] - Staining of rat spleen cells.
	Flow Cytometry: MHC Class II RT1B Antibody (OX-6) [NB100-65541] - Staining of rat spleen cells.
	Flow Cytometry: MHC Class II RT1B Antibody (OX-6) [NB100-65541] - Staining of rat spleen cells.
	Flow Cytometry: MHC Class II RT1B Antibody (OX-6) [NB100-65541] - Staining of rat spleen cells.

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Background

MHC Class II antigens are heterodimers consisting of one alpha chain(31-34kD) and one beta chain (26-29kD). The family of monoclonal antibodies (ER-TR 3, ER-TR 2, ER-TR 1) detect MHC class II antigens encoded by the murine Ia region of the H-2 complex, corresponding to the human HLA-DR region. MHC Class II antigens are a valuable tool for studying T helper cell interaction with class II positive antigen presenting cells (dendritic cells, B cells, macrophages) and offer new possibilities for studying the development of T helper cells since these antibodies also stain stromal cells in the thymus. MHC Class II antigens are also inducible on a number of other cells (endothelium and epithelial cells) by interferon gamma.

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