抗His Tag (AD1.1.10)抗体 | Anti-His Tag (AD1.1.10) Antibody

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	Western Blot: His Tag Antibody (AD1.1.10) [NB100-64768] - Analysis using the Biotin conjugate of NB100-64768. Detection of HIS tagged protein probed with mouse anti histidine tag : Biotin and visualised with Streptavidin : HRP.
	Flow Cytometry: His Tag Antibody (AD1.1.10) [NB100-64768] - Analysis using the FITC conjugate of NB100-64768. Staining of Human anti-human CD5 (HuCAL dHLX antibody with HIS tag) human peripheral blood lymphocytes, visualised with FITC conjugated Mouse anti-HIS tag antibody
	Western Blot: His Tag Antibody (AD1.1.10) [NB100-64768] - Drosophila, 20 ug whole cell lysate
	Western Blot: His Tag Antibody (AD1.1.10) [NB100-64768] - 6-Histidine Epitope Tag Antibody (AD1.1.10) [NB100-64768] - Analysis using the HRP conjugate of NB100-64768. Detection of His-Ubiquitin recombinant protein probed with Mouse anti Histidine Tag.
	Western Blot: His Tag Antibody (AD1.1.10) [NB100-64768] - Western blot analysis of 1) 6x-His tagged FLCN protein and 2) 6x-His tagged homer protein.
	Simple Western: His Tag Antibody (AD1.1.10) [NB100-64768] - Simple Western lane view shows a specific band for 6-His Epitope Tag in 0.1 mg/ml of rhEDG6 CHO S Tx lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

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Background

Epitope tagging is the procedure of inserting a short amino acid sequence into a protein within an expression vector via genetic engineering and it is a widely used technique in proteomics and various other biological sciences. The sequence of the tag is engineered using recombinant DNA technology, and grafted into the protein of interest by placing the encoded sequence of the epitope into a matching open reading frame. The antibody generated against this engineered epitope can recognize it, thereby enabling the tagged protein to be isolated, purified and visualized/analyzed by immunodetection methods. In other words, epitope tags represents an artificial epitope that is recognized by a common antibody and the antibodies recognizing a tag are useful for detecting proteins with low immunogenicity. 6-Histidine or H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate their purification and detection. Our 6-Histidine Epitope Tag antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells, and is useful for Western blot, IHC- paraffin embedded on frozen sections, ELISA, FLOW Cytometry and Immunoprecipitation applications.

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