抗VEGF R2/KDR/Flk-1抗体 | Anti-VEGF R2/KDR/Flk-1 Antibody

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	Western Blot: VEGF R2/KDR/Flk-1 Antibody [NB100-627] - Lane 1 is 20 ug, lane 2 is 40 ug and lane 3 is 50 ug of protein. KDR primary antibody was used at 1:500. The secondary was HRP goat anti-rabbit at 1:4,000. ECL detection was at 86 sec. This image was submitted via customer Review. *{Sheep WB}*
	Immunohistochemistry-Paraffin: VEGF R2/KDR/Flk-1 Antibody [NB100-627] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human placenta using VEGF R2 antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Syncitiotrophoblast staining was observed. Staining was performed by Histowiz.
	Western Blot: VEGF R2/KDR/Flk-1 Antibody [NB100-627] - Analysis of VEGF R2/KDR/Flk-1 in mouse brain tumor lysates using anti-VEGF R2/KDR/Flk-1 antibody. Image from verified customer review.
	Western Blot: VEGF R2/KDR/Flk-1 Antibody [NB100-627] - Detection of VEGFR2 in CSF-VEGF R2/KDR/Flk-1 transfected lysates using NB 100-627. ECL detection in 15 seconds. Lane 1 - 1:2,000 Lane 2 - 1:10,000.

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Background

VEGFs such as VEGFA, VEGFC, VEGFD etc. activate RTKs namely VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1), and VEGFR-3 (Flt-4), that regulates angiogenic as well as lymphangiogenic transduction signals. VEGF Receptor 2 (VEGFR2) is the most prominent cell-surface receptor for VEGF ligands and binding of VEGFs to isoform 1 leads to multiple signaling cascades activation. PLCG1 activation results in production of signaling molecules (diacylglycerol, inositol 1,4,5-trisphosphate etc) and PKC activation. VEGFR2 is involved in the activation of MAPK1/ERK2, MAPK3/ERK1, and MAPK as well as AKT signaling pathways. It facilitates phosphorylation of PIK3R1 (regulatory subunit of PI3K), cytoskeletal actin reorganization and PTK2/FAK1 activation. VEGFR2 is essential for VEGFA-mediated induction of NOS2/NOS3, leading to the production of NO by endothelial cells and is involved in phosphorylation of FYN, NCK1, NOS3, PIK3R1 and SRC. Isoforms -2 and -3 function as decoy receptors for VEGFs, and isoform -2 plays as negative regulator of VEGFA/VEGFC-mediated lymphangiogenesis by limiting free VEGFA and/or VEGFC levels alongwith preventing their binding to VEGFR3. Versatile signaling effects by VEGFR2 leads to its regulatory role in processes such as angiogenesis, vascular development, vascular permeability, embryonic hematopoiesis, and promotes proliferation, survival, migration and differentiation of endothelial cells alongwith facilitating reorganization of the actin cytoskeleton.

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