抗beta-Actin抗体 | Anti-beta-Actin Antibody

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	Immunocytochemistry/Immunofluorescence: beta-Actin Antibody [NB100-56874] - Actin was detected in NIH-3T3 cells fixed with methanol using rabbit anti-mouse Actin antibody (NB100-56874) at 1:100 dilution, overnight at 4C. Cells were stained using Northern Lights 557 conjugated anti-rabbit secondary antibodies (NL004) and counterstained with DAPI.
	Simple Western: beta-Actin Antibody [NB100-56874] - Simple Western lane view shows a specific band for Beta Actin in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.
	Western Blot: beta-Actin Antibody [NB100-56874] - Analysis using beta actin antibody: Lysate from A: human brain, B: mouse brain, C: rat brain, D: human lung and E: human spleen tissue probed using beta actin antibody at 0.25 ug/ml.
	Western Blot: beta-Actin Antibody [NB100-56874] - Analysis using beta actin antibody: Decreasing amounts of human ovary tissue lysate were probed using beta actin antibody at 0.25 ug/ml: A) 40 ug, B) 30 ug, C) 20 ug, and D) 10 ug per lane.
	Western Blot: beta-Actin Antibody [NB100-56874] - Analysis of HeLa, K562, A431, C2C12, C6, and Rat2 cell lines.
	Immunohistochemistry-Paraffin: beta-Actin Antibody [NB100-56874] - analysisof human spleen using antibody at 10 ug/ml.

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Background

Actin is a globular protein found in all eukaryotic cells (the only known exception being nematode sperm) where it may be present at concentrations of over 100 uM. It is the monomeric subunit of two types of filaments: microfilaments, one of the three major components of the cytoskeleton, and thin filaments, part of the contractile apparatus in muscle cells. Actin participates in many important cellular processes including muscle contraction, cell signaling, motility and division, cytokinesis, vesicle and organelle movement, and the establishment and maintenance of cell junctions and shape. In vertebrates, three main groups of actin isoforms, alpha, beta, and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cells as components of the cytoskeleton, and as mediators of internal cell motility (Doherty GJ, et al 2008). Among these actins, beta actin, which is expressed constantly, and at high levels, in all the tissue types used in protein research except muscle (skeletal and cardiac). Beta actin acts as a useful loading control in Western blot analysis. Loading controls serve a number of purposes; they ensure the protein of interest has been correctly loaded on the gel, that it is being transferred correctly, and that all reagents are functioning normally. Loading controls can also be used to compare amounts of protein tested (or loaded) per gel lane in western blotting. While very useful when comparing lysates of the same origin (for example transfected and untransfected HEK 293s), comparing lysates from different tissues can be complex. Actin is almost universally expressed, but different tissues express different amounts. In western blotting, an actin antibody titered to the detection limit can show these differences, while an actin antibody used well above the detection limit can show a response that appears equally robust in all samples probed. Therefore caution must be used when using actin or another 'loading control antibody.'

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