抗TRANCE/TNFSF11/RANK L (12A380)抗体 | Anti-TRANCE/TNFSF11/RANK L (12A380) Antibody

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	Western Blot: TRANCE/TNFSF11/RANK L Antibody (12A380) [NB100-56748] - Analysis of recombinant RANKL (partial) probed with RANKL antibody at 1 ug/ml.
	Flow Cytometry: TRANCE/TNFSF11/RANK L Antibody (12A380) [NB100-56748] - Analysis using Azide Free version of NB100-56748. RAW cells using 2 ug of PE conjugated antibody. Shaded histogram represents cells without antibody; green represents isotype control antibody; purple represents antibody.
	Immunohistochemistry-Paraffin: TRANCE/TNFSF11/RANK L Antibody (12A380) [NB100-56748] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human tonsil using TRANCE (12A380) antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining was observed. Staining was performed by Histowiz.
	Flow Cytometry: TRANCE/TNFSF11/RANK L Antibody (12A380) [NB100-56748] - Analysis using the Alexa Fluor (R) 647 conjugate of NB100-56748. Staining of RANKL in untreated mouse splenocytes (green) and 72 hour ConA-stimulated mouse splenocytes (red) using this antibody. 1 ug of RANKL antibody was used for this assay.
	Flow Cytometry: TRANCE/TNFSF11/RANK L Antibody (12A380) [NB100-56748] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-56748. Staining of RANKL in untreated mouse splenocytes (green) and 72 hour ConA-stimulated mouse splenocytes (red) using this antibody. 1 ug of RANKL antibody was used for this assay.

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Background

TRANCE/OPGL/RANKL/ODF is a recently identified member of tumor necrosis factor family. TRANCE (TNF-related activation-induced cytokine receptor) or RANKL {receptor activator of NF-kB (RANK) ligand} has been implicated in interactions between T cells and dendritic cells. RANK ligand (RANKL/TRANCE) binds to RANK on dendritic cells, upregulates the expression of anti-apoptotic protein BcL-XL suggesting a role in dendritic cell survival. TRANCE/RANKL is also important in T- and B-cell maturation. As OPGL/ODF (Osteoprotegerin Ligand/Osteoclast differentiation factor), the same protein can both activate mature osteoclasts and mediate osteoclastogenesis. OPGL/TRANCE deficient mice show severe osteoporesis and complete absence of osteoclasts as a result of lack of osteogenesis.

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