抗TLR4 (HTA125) - Azide Free抗体 | Anti-TLR4 (HTA125) - Azide Free Antibody

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	Flow Cytometry: TLR4 Antibody (HTA125) - Azide Free [NB100-56727] - Analysis using Azide/BSA FREE version of NB100-56727. Cell surface analysis of TLR4 in stable HEK293/hTLR4 cells using this antibody. Secondary antibody goat anti-mouse IgG-PE at 0.25ug/10^6 cells. Both stable HEK293/hTLR4 and HEK293/Vector cell lines were equally stained.
	Flow (Cell Surface): TLR4 Antibody (HTA125) - Azide Free [NB100-56727] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-56727. Staining of TLR4 on PBMC using this antibody at 1 ug/10^6 cells. Green represents isotype control this antibody; red represents anti-TLR4 antibody.
	Flow (Cell Surface): TLR4 Antibody (HTA125) - Azide Free [NB100-56727] - Analysis using the Alexa Fluor (R) 647 conjugate of NB100-56727. Staining of TLR4 in stable HEK293/hTLR4 cells using this antibody at 1ug/10^6 cells. Green represents HEK293/Vector cells; red represents HEK293/hTLR4 cells.
	Flow (Cell Surface): TLR4 Antibody (HTA125) - Azide Free [NB100-56727] - Analysis using the Alexa Fluor (R) 647 conjugate of NB100-56727. Staining of TLR4 on PBMC using this antibody at 1 ug/10^6 cells. Green represents isotype control this antibody; red represents anti-TLR4 antibody.
	Flow (Intracellular): TLR4 Antibody (HTA125) - Azide Free [NB100-56727] - Analysis using the PE conjugate of NB100-56727. Staining of TLR4 in human PBMCs using NB100-56062 at 0.5 ug/10^6 cells. The shaded histogram represents cells without anti-TLR4 antibody; green represents the isotype control; red represents Nb100-56062, ant
	Flow (Cell Surface): TLR4 Antibody (HTA125) - Azide Free [NB100-56727] - Analysis using the PE conjugate of NB100-56727. Staining of TLR4 on ThP1 cells using TLR4 antibody at 2 ug/10^6 cells. The shaded histogram represents ThP1 cells only, green represents isotype control antibody, and red represents TLR4 antibody.
	Flow Cytometry: TLR4 Antibody (HTA125) - Azide Free [NB100-56727] - Analysis using Azide/BSA FREE version of NB100-56727. Analysis of TLR4 using NB100-56723 on PBMCs at 2ug/ 1X10^6 cells. M1 gate are unstained PBMC.
	Flow Cytometry: TLR4 Antibody (HTA125) - Azide Free [NB100-56727] - Analysis using Azide/BSA FREE version of NB100-56727. Cell surface analysis of TLR4 in stable HEK293/hTLR4 cells using NB100-56723 at 1 ug/10^6 cells. Secondary antibody Goat anti-Mouse IgG-PE at 0.25ug/10^6 cells.
	Flow Cytometry: TLR4 Antibody (HTA125) - Azide Free [NB100-56727] - Analysis using Azide/BSA FREE version of NB100-56727. Staining of human peripheral blood monocytes with Mouse anti Human CD284.
	Blocking/Neutralizing: TLR4 Antibody (HTA125) - Azide Free [NB100-56727] - Validation of the neutralizing effect of this antibody on TLR4 activity. The TLR4/MD-2/CD14/IL-8 Prom/LUCPorter HeLa cells were plated in 96-well white plates at 1 x 10^5 cells/well. After 16 h, cells were preincubated with different amounts of this antibody for 1 h and then stimulated with 10 ng/ml LPS for 6 h. Luciferase activity was then directly analyzed using the one-step luciferase reporter assay reagent. Data Summary: It inhibited LPS-mediated TLR4 activity in the TLR4/MD-2/CD14/IL-8 Prom/LUCPorter cell line.

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Background

The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). Among this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling (4,5). The TLR4 cDNA codes for a protein consisting of 799 amino acids with approximate molecular weight of 88 kDa (6).

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