抗RelA/NFkB p65 (112A1021)抗体 | Anti-RelA/NFkB p65 (112A1021) Antibody

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	Western Blot: RelA/NFkB p65 Antibody (112A1021) [NB100-56712] - Lysates of HeLa human cervical epithelial carcinoma cell line and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 ug/mL mouse anti-RelA/NFkB p65 monoclonal (NB100-56712, Novus Biologicals), followed by 1:2000 dilution of the appropriate HRP-conjugated secondary antibody, donkey anti-mouse IgG (HAF018).
	Immunohistochemistry-Paraffin: RelA/NFkB p65 Antibody (112A1021) [NB100-56712] - Analysis using Azide Free version of NB100-56712. Formalin-fixed, paraffin-embedded ovarian cystadenocarcinoma probed with p65 antibody at 5 ug/ml. Human tissue TMA was used for this test. Staining of formalin-fixed tissues is enhanced by boiling tissue s
	Flow (Intracellular): RelA/NFkB p65 Antibody (112A1021) [NB100-56712] - Analysis using Azide Free version of NB100-56712. Intracellular staining of 293 HEK cells using 0.5 ug of p65 antibody. Green histogram represents the isotype control (p65) antibody. this antibody was used for this test, and an anti-mouse IgG PE conjugate
	Western Blot: RelA/NFkB p65 Antibody (112A1021) [NB100-56712] - Analysis of NF-kB (p65) using NB100-56055 (biotinylated version of Clone 112A1021) at 2 ug/ml in 30 ug of A) Ramos, B) Daudi, C) HeLa and D) mouse NIH 3T3 cell lysate.
	Western Blot: RelA/NFkB p65 Antibody (112A1021) [NB100-56712] - Analysis of Biotin conjugate of NB100-56712. OfRelA/NFkB p65 Antibody (112A1021) [Biotin]using NB100-56055 at 2 ug/ml in 30 ug of A) Ramos, B) Daudi, C) HeLa and D) mouse NIH 3T3 cell lysate.
	Western Blot: RelA/NFkB p65 Antibody (112A1021) [NB100-56712] - Analysis using Azide Free version of NB100-56712. P65 using p65 antibody at 2 ug/ml in 30 ug of A) Ramos, B) Daudi, C) HeLa and D) mouse NIH 3T3 cell lysate.
	Immunohistochemistry-Paraffin: RelA/NFkB p65 Antibody (112A1021) [NB100-56712] - Formalin-fixed, paraffin-embedded ovarian cystadenocarcinoma probed with p65 antibody at 5 ug/ml. Human tissue TMA was used for this test. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
	Flow Cytometry: RelA/NFkB p65 Antibody (112A1021) [NB100-56712] - Intracellular staining of 293 HEK cells using 0.5 ug of p65 antibody. Green histogram represents the isotype control (p65) antibody. This NFkB p65 antibody was used for this test with an anti-mouse IgG PE conjugated secondary antibody.
	Simple Western: RelA/NFkB p65 Antibody (112A1021) [NB100-56712] - Simple Western lane view shows a specific band for NFkB p65 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
	Simple Western: RelA/NFkB p65 Antibody (112A1021) [NB100-56712] - Analysis using Azide Free version of NB100-56712. Simple Western lane view shows a specific band for NFkB p65 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

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Background

NF-kB (nuclear factor kB) regulates the expression of a large number of genes that play critical roles in apoptosis, viral replication, tumorigenesis, various autoimmune diseases and inflammation. The active nuclear form of the NF-kB transcription factor complex is composed of two DNA binding subunits, NF-kB p65 and p50, both of which share extensive N-terminal sequence homology with the v-rel oncogene product. N-terminal regions of p50 and p65 are critical for DNA binding and help determine the DNA-binding specificities of p50 and p65.

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