抗IKK alpha (14A231)抗体 | Anti-IKK alpha (14A231) Antibody

• 価格
• Image
• Background

価格

目次に戻る

Image

	Western Blot: IKK alpha Antibody (14A231) [NB100-56704] - Analysis using Azide Free version of NB100-56704. IKK in Daudi cell lysate using IKKa monoclonal antibody at 1 ug/ml.
	Immunocytochemistry/Immunofluorescence: IKK alpha Antibody (14A231) [NB100-56704] - Antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). An antibody dilution of 1:10 was used. Image objective 40x.
	Immunohistochemistry-Paraffin: IKK alpha Antibody (14A231) [NB100-56704] - Analysis using Azide Free version of NB100-56704. Formalin-fixed, paraffin-embedded human kidney stained with IKKa antibody at 5 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10
	Flow Cytometry: IKK alpha Antibody (14A231) [NB100-56704] - Analysis using Azide Free version of NB100-56704. Intracellular staining of HEK293 cells using 0.5 ug of IKKa antibody (red) and isotype control (green). Intracellular flow kit was used for this test, and an anti-mouse IgG1 PE conjugated secondary antibod
	Western Blot: IKK alpha Antibody (14A231) [NB100-56704] - Analysis of IKK alpha in Daudi cell lysate using IKK alpha antibody at 1 ug/ml.
	Western Blot: IKK alpha Antibody (14A231) [NB100-56704] - Total protein from various Human, Mouse and Rat cell lines were separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-IKK-alpha in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
	Flow Cytometry: IKK alpha Antibody (14A231) [NB100-56704] - Intracellular staining of HEK293 cells using 0.5 ug of IKK alpha antibody. Green histogram represents the isotype control antibody, red represents the IKK alpha antibody. Intracellular flow kit was used for this test, and an anti-mouse IgG1 PE conjugated secondary antibody.
	Simple Western: IKK alpha Antibody (14A231) [NB100-56704] - Simple Western lane view shows a specific band for IKK alpha in 0.5 mg/ml of Daudi (left) and U937 (right) lysate. This exeriment was performed under reducing conditions using the 12-230 kDa separation system.

目次に戻る

Background

NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g. In vitro, IKK1/a and IKK2/b can form homo- and heterodimers that can phosphorylate IkBs at the regulatory serine residues directly. IKK1/a and IKK2/b are phosphorylated by NF-kB-inducing kinase (NIK) and MAP kinase kinase kinase-1 (MEKK1), respectively. Targeted disruption of IKK1/a gene in mice results in skin and limb abnormalities and death of newborns.

目次に戻る