抗TLR4 (76B357.1)抗体 | Anti-TLR4 (76B357.1) Antibody

• 価格
• Image
• Background

価格

目次に戻る

Image

	Western Blot: TLR4 Antibody (76B357.1) [NB100-56566] - Analysis using 2 ug/ml on (A) human intestine and 6 ug/ml on (B) mouse intestine and C) rat intestine lysate.
	Immunohistochemistry-Paraffin: TLR4 Antibody (76B357.1) [NB100-56566] - Formalin-fixed, paraffin-embedded human skin stained with at 5 ug/ml, peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
	Flow (Intracellular): TLR4 Antibody (76B357.1) [NB100-56566] - Analysis using PE conjugate of NBP2-27149. An intracellular stain was performed on Jurkat cells with TLR4 antibody (76B357.1) NBP2-27149PE (blue) and an isotype control MAB004 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.
	Flow Cytometry: TLR4 Antibody (76B357.1) [NB100-56566] - Flow cytometric analysis of formaldehyde fixed THP-1 cells (human monocytic leukemia cells) using 2 ug/10^6 cells TLR4 antibody (clone 76B357.1) with detection employing a donkey anti-mouse IgG (H+L) cross adsorbed secondary antibody, (DyLight 488 conjugated). Isotype control samples incubated with mouse IgG2b isotype control antibody were processed in parallal under the same assay conditions.
	Flow Cytometry: TLR4 Antibody (76B357.1) [NB100-56566] - Analysis using the Alexa Fluor (R) 647 conjugate of NBP2-27149. TLR4 expression on monocytes from human peripheral blood: PBMC were stained, in a 2 color flow test, with CD14 PE this antibody and 1 ug of either isotype control (Left, ) or TLR4-Alexa Fluor 647 (right). PPI negative, CD14+ cells were gated for analysis.
	Flow Cytometry: TLR4 Antibody (76B357.1) [NB100-56566] - Flow analysis of 10^6 ThP1 cells using 5 ug. Shaded histogram shows ThP1 cells without anti-TLR4 antibody; green represents isotype control; red represents anti-TLR4 antibody.
	Flow (Cell Surface): TLR4 Antibody (76B357.1) [NB100-56566] - Analysis using the FITC conjugate of NBP2-27149. Staining of cell surface TLR4 in human monocyte-derived dendritic cells using this antibody at 1:200 (2.5 ug). Black and green open histograms represent unstained cells and negative control (anti-human CD14
	Immunohistochemistry-Paraffin: TLR4 Antibody (76B357.1) [NB100-56566] - Analysis of formalin-fixed paraffin-embedded tissue section of normal human skin at 5 ug/ml. Membrane-cytoplasmic immunopositivity of TLR4 was primarily observed in the pigmented basel cells and the adjacent keratinocytes in the epidermal layer.
	Immunohistochemistry-Frozen: TLR4 Antibody (76B357.1) [NB100-56566] - This image is TLR4(green) and nucleus(blue) at area postrema of the adult male mouse brai n, x20 magnification. Primary antibody diluted 1:500. This image was submitted via customer Review.
	Flow (Intracellular): TLR4 Antibody (76B357.1) [NB100-56566] - An intracellular stain was performed on Jurkat cells with TLR4 antibody (76B357.1) NBP2-27149AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647. Image from the Alexa Fluor 647 version of this antibody.
	Immunohistochemistry-Paraffin: TLR4 Antibody (76B357.1) [NB100-56566] - Analysis of formalin-fixed paraffin-embedded tissue section of normal human breast at 5 ug/ml. The ductal/acinar epithelial cells and the myoepithelial cells showed specific membrane-cytoplasmic positivity for TLR4. Mild immunoreactivity of TLR4 was observed in blood vessels and the intra-lobular connective tissue also.
	Immunohistochemistry-Paraffin: TLR4 Antibody (76B357.1) [NB100-56566] - Analysis of formalin-fixed paraffin-embedded tissue section of human esophageal squamous cell carcinoma (SCC) using 5 ug/ml. Membrane-cytoplasmic immunopositivity was observed in SCC cells while the tumor stroma was largely negative for TLR4.
	Immunohistochemistry-Paraffin: TLR4 Antibody (76B357.1) [NB100-56566] - Analysis of formalin-fixed paraffin-embedded tissue section of malignant stromal tumor of small intestine from human at 5 ug/ml. The representative image shows a membrane-cytoplasmic staining pattern of TLR4 in the cancer cells.
	Immunohistochemistry-Paraffin: TLR4 Antibody (76B357.1) [NB100-56566] - Analysis of Rat's salivary gland tissue section at 1:100 dilution. The antibody generated a membrane-cytoplasmic staining in the tissue with stronger signal in ductal epithelial cells.
	Immunohistochemistry-Paraffin: TLR4 Antibody (76B357.1) [NB100-56566] - Analysis of TLR4 in paraffin-embedded formalin-fixed human colon tissue using an isotype control (top) and NB100-56566 (bottom) at 5 ug/ml.
	Immunohistochemistry-Paraffin: TLR4 Antibody (76B357.1) [NB100-56566] - Formalin-fixed, paraffin-embedded human testis tissue stained using 5 ug/ml.
	Flow Cytometry: TLR4 Antibody (76B357.1) [NB100-56566] - Surface staining of mouse splenocytes using TLR4 antibody (0.5 ug/10^6 cells) and a PE-conjugated secondary this antibody. Shaded histogram represents cells without TLR4 antibody; green represents isotype control (BD Pharmingen) red represents TLR4 antibody.
	Flow Cytometry: TLR4 Antibody (76B357.1) [NB100-56566] - Analysis of FITC conjugate of NB100-56566. Cell surface TLR4 in human monocyte-derived dendritic cells using this antibody at 1:200 (2.5 ug). Black and green open histograms represent unstained cells and negative control (anti-human CD14 FITC, respectivel
	Flow Cytometry: TLR4 Antibody (76B357.1) [NB100-56566] - Analysis using the FITC conjugate of NBP2-27149. TLR4 expression on monocytes from human peripheral blood: PBMC were stained, in a 2 color flow test, with CD14 PE and 1 ug of either isotype control (Left, ) or TLR4-FITC (Right, this antibody. PPI negative, CD14+ cells were gated for analysis.

目次に戻る

Background

The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. Among this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling. The TLR4 cDNA codes for a protein consisting of 799 amino acids with approximate molecular weight of 88 kDa.

目次に戻る