抗IKK beta (10A9B6)抗体 | Anti-IKK beta (10A9B6) Antibody

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	Western Blot: IKK beta Antibody (10A9B6) [NB100-56513] - Analysis using Azide Free version of NB100-56513. Daudi cell lysate using IKK beta antibody at 5 ug/ml.
	Flow (Intracellular): IKK beta Antibody (10A9B6) [NB100-56513] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-56513. Staining of IKK in HEK 293 cells using 0.1 ug of this antibody. Green histogram represents the isotype control, red represents the IKK antibody.
	Western Blot: IKK beta Antibody (10A9B6) [NB100-56513] - IKK beta in Daudi cell lysate using IKK beta antibody at 5 ug/ml.
	Western Blot: IKK beta Antibody (10A9B6) [NB100-56513] - Analysis using Azide Free version of NB100-56513. Antibodoy was used to immunoprecipitate IKKb from 1x10^6 Daudi cells and the immuno-precipitated protein was detected by western blotting using NB100-56509. IKKb was detected as a protein of ~87 kD.
	Simple Western: IKK beta Antibody (10A9B6) [NB100-56513] - Simple Western lane view shows a specific band for IKK beta in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. *Non-specific interaction with the 230 kDa standard may be seen with this antibody.
	Simple Western: IKK beta Antibody (10A9B6) [NB100-56513] - Analysis using Azide Free version of NB100-56513. Lane view shows a specific band for IKK beta in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. *Non-specific interaction with the

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Background

NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g. IKK1/a and IKK2/b are phosphorylated by NF-kB-inducing kinase (NIK) and MAP kinase kinase kinase-1 (MEKK1), respectively. Recent studies have shown that IKK2/b, not IKK1/a, is the target proinflammatory stimuli. Targeted disruption of IKK2/b gene in mice also results in extensive liver damage from apotopsis and death as embryo.

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