抗PCNA抗体 | Anti-PCNA Antibody

掲載日情報:2018/07/09 現在Webページ番号:46970

世界最大級の抗体製品数を取り扱うNovus Biologicals社のPCNAに対する抗体(anti-PCNA | antibody PCNA)です。Novus Biologicals社の抗体は数多くの学術論文で使用実績があります。

本製品は研究用です。研究用以外には使用できません。

価格表左の「文献」アイコンから、使用文献情報一覧が表示できます。

価格

[在庫・価格 :2025年07月17日 16時55分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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納期 文献数
Anti-PCNA, Rabbit-Poly
3~4週間 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 5
説明文
Keywords:cyclin|DNA polymerase delta auxiliary protein|MGC8367|proliferating cell nuclear antigen
Genbank No: 5111
Protein Accession No: P12004
法規制等
保存条件 法規備考
抗原種 Human 免疫動物 Rabbit クラス IgG 標識 Unlabeled
交差性 Human/Mouse 適用 FCM,IHC,IP,Western Blot
クロナリティ Polyclonal フォーマット 性状 Antigen Affinity Purified 吸収処理
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[在庫・価格 :2025年07月17日 16時55分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-PCNA, Rabbit-Poly

文献数: 5

説明文 Keywords:cyclin|DNA polymerase delta auxiliary protein|MGC8367|proliferating cell nuclear antigen
Genbank No: 5111
Protein Accession No: P12004
法規制等
保存条件 法規備考
抗原種 Human 免疫動物 Rabbit
交差性 Human/Mouse 適用 FCM,IHC,IP,Western Blot
標識 Unlabeled 性状 Antigen Affinity Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事
関連記事



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Image

Biological Strategies Validation. Western Blot: PCNA Antibody [NB100-456] - Detection of Human and Mouse PCNA by Western Blot. Samples: Whole cell lysate (50 ug) from 293T and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PCNA antibody NB100-456 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry: PCNA Antibody [NB100-456] - Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti- PCNA used at a dilution of 1:10,000 (0.1ug/ml). Detection: DAB
Flow Cytometry: PCNA Antibody [NB100-456] - Detection of PCNA Versus DNA Content. Asynchronous Jurkat cells were fixed and permeabilized in a sequential treatment of FACS buffer (PBS, 0.5% triton-X-100, 0.5mM EDTA, 1% BSA) and 100% methanol. 1 X106 cells were stained with 0.03 ug anti-PCNA. Secondary detection was performed with FITC conjugated Goat F(ab')2 anti-rabbit antibody], and DNA stained with PI.
Western Blot: PCNA Antibody [NB100-456] - A431 whole cell lysates
Biological Strategies Validation. Western Blot: PCNA Antibody [NB100-456] - Analysis using the Biotin conjugate of NB100-456. Detection ofHeLa (50, 15, and 5 ug). Antibody used at 1.0 ug/ml. Detection: Streptavidin-HRP and chemiluminescence with an exposure time of 3 seconds.
Proximity Ligation Assay: PCNA Antibody [NB100-456] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RFC5 (NB100-235) and rabbit anti-human PCNA (NB100-456). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.
Proximity Ligation Assay: PCNA Antibody [NB100-456] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human DMAP1 (NB100-256) and rabbit anti-human PCNA (NB100-456). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.
Proximity Ligation Assay: PCNA Antibody [NB100-456] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human CHK1 (NB100-274) and rabbit anti-human PCNA (NB100-456). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.
Proximity Ligation Assay: PCNA Antibody [NB100-456] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human SMARCA4 (NBP2-22234) and rabbit anti-human PCNA (NB100-456). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.
Proximity Ligation Assay: PCNA Antibody [NB100-456] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RFC4 (NB100-233) and rabbit anti-human PCNA (NB100-456). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.
Proximity Ligation Assay: PCNA Antibody [NB100-456] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RPA2 (NBP2-22232) and rabbit anti-human PCNA (NB100-456). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.
Proximity Ligation Assay: PCNA Antibody [NB100-456] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RFC2 (NB100-232) and rabbit anti-human PCNA (NB100-456).Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.
Proximity Ligation Assay: PCNA Antibody [NB100-456] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human MSH6 (NB100-328) and rabbit anti-human PCNA (NB100-456). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.
Proximity Ligation Assay: PCNA Antibody [NB100-456] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RFC1 (NB100-230) and rabbit anti-human PCNA (NB100-456). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.


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Background

PCNA is a homotrimeric protein that is involved in DNA replication and DNA repair. It serves as a processivity factor for DNA polymerase delta. It has also been shown to interact with several other proteins that are involved in DNA replication and repair, including replication factor C, DNA ligase I, XPG, FEN1 and p21. This particular antiserum was raised against the interdomain connector loop region of PCNA, which is the region of PCNA through which many of the protein-protein interactions occur.

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