抗FoxP3抗体 | Anti-FoxP3 Antibody

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	Immunohistochemistry-Paraffin: FoxP3 Antibody [NB100-39002] - Mouse spleen stained with FoxP3 antibody. Image from verified customer review.
	Immunohistochemistry-Paraffin: FoxP3 Antibody [NB100-39002] - IHC analysis of a formalin fixed paraffin embedded tissue section of human tonsil using 1:200 dilution of rabbit anti-FoxP3 antibody. The staining was developed with HRP labeled anti-rabbit IgG secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This FoxP3 antibody generated an expected and a specific nuclear staining of FOXP3 in a subset of cells which are potentially the Treg cells.
	Immunohistochemistry: FoxP3 Antibody [NB100-39002] - FOXP3 Antibody [NB100-39002] - FOXP3 antibody was tested in mouse spleen using DAB with hematoxylin counterstain.
	Immunohistochemistry-Paraffin: FoxP3 Antibody [NB100-39002] - IHC analysis of a formalin fixed paraffin embedded tissue section of human tonsil using 1:200 dilution of rabbit anti-FoxP3 antibody. The staining was developed with HRP labeled anti-rabbit IgG secondary antibody and DAB reagent, and nuclei of cells were counterstained with hematoxylin. This FoxP3 antibody generated an expected and a specific nuclear staining of FOXP3 in a subset of cells which are potentially the Treg cells.
	Expression of Tagged Proteins Validation. Western Blot: FoxP3 Antibody [NB100-39002] - FOXP3 Antibody [NB100-39002] - Detection of immunoprecipitated FOXP3 from HA-tagged FOXP3 transfected Jurkat T antigen cells.
	Flow Cytometry: FoxP3 Antibody [NB100-39002] - FOXP3 Antibody [NB100-39002] - Transfectants from A were permeabilized and stained with anti-Foxp3 antibody and FITC-conjugated secondary antibody. Cells were analyzed by flow cytometry. Percentage of positively stained cells are shown.
	Genetic Strategies Validation. Western Blot: FoxP3 Antibody [NB100-39002] - FOXP3 Antibody [NB100-39002] - Jurkat Tag cells were transfected with the expression vector (vector) or with the Foxp3 expression construct (Foxp3). 24 hours after transfection, cells were lysed and analyzed by Western blot with anti-Foxp3 antiserum (1:2000 dilution).
	Genetic Strategies Validation. Immunocytochemistry/Immunofluorescence: FoxP3 Antibody [NB100-39002] - FOXP3 Antibody [NB100-39002] - The same transfectants were stained with anti-Foxp3 antibody and HRPO-conjugated (upper panel) or Cy3-conjugated (lower panels) secondary antibody. Representative images (x100) are shown.

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Background

FOXP3 (a 381 amino acid protein) is a member of the forkhead/winged-helix family of transcriptional regulators and is highly conserved across mammals. FOXP3 is essential for normal immune homeostasis. FOXP3 is stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene and a more specific marker of regulatory T cells than other T cells.

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